Rabbit Anti-StAR antibody | Sunlong Medical

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StARD1; Cholesterol trafficker; Luteinizing hormone induced protein; Mitochondrial steroid acute regulatory protein; StAR related lipid transfer (START) domain containing 1; StARD1; START domain containing protein 1; Steroidogenic Acute Regulatory Protein

Cat:

SL3570R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human StAR/StARD1:101-200/285

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3μg /TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Ovary (Mouse) Lysate at 40 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: Kidney (Mouse) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: K562 (Human) Cell Lysate at 30 ugLane 6: THP-1 (Human) Cell Lysate at 30 ugLane 7: U937 (Human) Cell Lysate at 30 ugLane 8: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-StAR (SL3570R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 27 kDSample:K562(human) cell Lysate at 30 ugPrimary: Anti-StAR (SL3570R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32kDObserved band size: 32 kDSample:Adrenal gland(mouse) Lysate at 40 ugPrimary: Anti-StAR (SL3570R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32kDObserved band size: 32 kD Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (SL3570R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-StAR antibody (SL3570R),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008].

Function:
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.

Subunit:
May interact with TSPO.

Subcellular Location:
Mitochondrion.

DISEASE:
Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma rennin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.

Similarity:
Contains 1 START domain.

SWISS:
P49675

Gene ID:
6770

Database links:

Entrez Gene: 6770 Human

Entrez Gene: 20845 Mouse

Entrez Gene: 25557 Rat

Omim: 600617 Human

SwissProt: P49675 Human

SwissProt: P51557 Mouse

SwissProt: P97826 Rat

Unigene: 521535 Human

Unigene: 293314 Mouse

Unigene: 11399 Rat

Picture

Sample:
K562(human) cell Lysate at 30 ug
Primary: Anti-StAR (SL3570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32kD
Observed band size: 32 kD

Sample:
Adrenal gland(mouse) Lysate at 40 ug
Primary: Anti-StAR (SL3570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32kD
Observed band size: 32 kD

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (SL3570R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-StAR antibody (SL3570R),Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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Specific References (11) | SL3570R has been referenced in 11 publications.

[IF=2.74] Hua Yang. et al. SMAD2 regulates testicular development and testosterone synthesis in Hu sheep. Theriogenology. 2021 Oct;174:139 WB ; Sheep.

PubMed:34454319

[IF=3.75] Lu-ming Wu. et al. Dulaglutide, a long-acting GLP-1 receptor agonist, can improve hyperandrogenemia and ovarian function in DHEA-induced PCOS rats. Peptides. 2021 Nov;145:170624 WB ; Rat.

PubMed:34375684

[IF=2.984] Junjie Hu. et al. Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis. Gene. 2021 Jul;790:145699 WB,IHC ; Sheep.

PubMed:3396876

[IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017). IHSLCP ; Mouse.

PubMed:29202267

[IF=3.813] Wenqian Xie. et al. Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus). J Steroid Biochem. 2020 Nov;204:105766 IHC ; Muskrat.

PubMed:32991988

[IF=2.094] Xiaodan Li. et al. Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in vitro. Theriogenology. 2021 May;166:9 WB ; Sheep.

PubMed:33667862

[IF=0.607] Yang F et al. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science.2019 Feb. WB ; Chicken.

PubMed:doi:10.1590/366-9061-2018-0835

[IF=2.638] An SY et al. MiR-1197-3p regulates testosterone secretion in goat Leydig cells via targeting PPARGC1A. Gene. 2019 May 31;710:131-139. WB ; Goat.

PubMed:31158446

[IF=6.54] Cook et al. Ethanol alters local cellular levels of (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP) independent of the adrenals in subcortical brain regions. (2014) Neuropsychopharmacology. 39:1978-87 IF ; Rat.

PubMed:24561363

[IF=1.84] Shi, Lei, et al. "Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig cells in vitro." Theriogenology 93 (2017): 24-32. WB ; Sheep.

PubMed:28257863

[IF=1.84] Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016). IHSLCP ; Sheep.

PubMed:28043364