Sample: Heart (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-phospho-PPAR alpha (Ser12) (SL4055R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Sample:
Liver (Rat) Lysate at 40 ug
Primary: Anti-phospho-PPAR alpha (Ser12) (SL4055R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Colon carcinoma (Human) Lysate at 40 ug
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- phospho-PPAR alpha (Ser12) (SL4055R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Mouse) Lysate at 40 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Lane 6: Jurkat (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-PPAR alpha (Ser12) (SL4055R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 50 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PPAR alpha(Ser12) Polyclonal Antibody, Unconjugated(SL4055R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
phosphopeptide
non phosphopeptide
|