Sample: U937 Cell (Human) Lysate at 40 ug
Primary: Anti-ICAM1 (SL4618R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 60 kD
Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti- ICAM1 (SL4618R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample: Raji Cell (Human) Lysate at 40 ug
Primary: Anti-ICAM1 (SL4618R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1) Polyclonal Antibody, Unconjugated (SL4618R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): U937 (fixed with 2% paraformaldehyde for 10 min at room temperature).
Primary Antibody (green line): Rabbit Anti-ICAM1 antibody (SL4618R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
|