Rabbit Anti-CD40/TNFRSF5 antibody

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Tumor necrosis factor receptor superfamily, member 5; TNFRSF5; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40 antigen (TNF receptor superfamily member 5); CD40 antig

Cat:

SL2929R

Species Reactivity：

Human,Mouse,Rat,Rabbit,(predicted: Chicken,Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human CD40/TNFRSF5.:201-277/277<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Jurkat(Human) Cell Lysate at 30 ugRaw264.7(Mouse) Cell Lysate at 30 ugPrimary: Anti-CD40(SL2929R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 52 kDSample: Lane 1: Spleen (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugLane 3: Lymph node (Rat) Lysate at 40 ugPrimary: Anti-CD40/TNFRSF5 (SL2929R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44-50 kDObserved band size: 50 kDSample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti-CD40(SL2929R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 52 kDParaformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD40) Polyclonal Antibody, Unconjugated (SL2929R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Subcellular Location:
Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted.

Tissue Specificity:
B-cells and in primary carcinomas.

Similarity:
Contains 4 TNFR-Cys repeats.

SWISS:
P25942

Gene ID:
958

Database links:

Entrez Gene: 958 Human

Entrez Gene: 21939 Mouse

Entrez Gene: 171369 Rat

Omim: 109535 Human

SwissProt: P25942 Human

SwissProt: P27512 Mouse

Unigene: 472860 Human

Unigene: 271833 Mouse

Picture

Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Rat) Lysate at 40 ug
Primary: Anti-CD40/TNFRSF5 (SL2929R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44-50 kD
Observed band size: 50 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-CD40(SL2929R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD40) Polyclonal Antibody, Unconjugated (SL2929R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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