Rabbit Anti-RAB9 antibody | Sunlong Medical

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DmRab9; Rab 9; RAB 9A; RAB9 member RAS oncogene family; RAB9A; RAB9A member RAS oncogene family; RAS ASSOCIATED PROTEIN RAB9; Ras related protein Rab 9A; Sid6061p; Sid99; RAB9A_HUMAN.

Cat:

SL6177R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human RAB9:65-170/201

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: K562(Human) Cell Lysate at 30 ugPrimary: Anti-RAB9 (SL6177R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 23 kDSample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti-RAB9 (SL6177R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 23 kDSample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti-RAB9 (SL6177R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 23 kDParaformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB9) Polyclonal Antibody, Unconjugated (SL6177R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-RAB9 Polyclonal Antibody, Unconjugated(SL6177R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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RAB proteins are GTPases that regulate vesicular trafficking and reside in specific intracellular compartments. RAB9 has been localized to components of the endocytic/exocytic pathway. It has been implicated in the recycling of membrane receptors, such as the mannose 6-phosphate receptor from early endosomes to the trans Golgi network.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Endoplasmic reticulum membrane (Potential). Golgi apparatus membrane (Potential). Late endosome.

Similarity:
Belongs to the small GTPase superfamily. Rab family.

SWISS:
P51151

Gene ID:
9367

Database links:

Entrez Gene: 511776 Cow

Entrez Gene: 403947 Dog

Entrez Gene: 9367 Human

Entrez Gene: 56382 Mouse

Entrez Gene: 84589 Rat

Omim: 300284 Human

SwissProt: P2888 Dog

SwissProt: P51151 Human

SwissProt: Q9R0M6 Mouse

SwissProt: Q99P75 Rat

Unigene: 495704 Human

Unigene: 721680 Human

Unigene: 25306 Mouse

Unigene: 35289 Rat

Picture

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-RAB9 (SL6177R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-RAB9 (SL6177R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD

Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB9) Polyclonal Antibody, Unconjugated (SL6177R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-RAB9 Polyclonal Antibody, Unconjugated(SL6177R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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