ASB10 is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. SOCS boxes are carboxy terminal regions of homology found in the suppressor of cytokine signaling (SOCS) family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases. The SOCS box couples the suppressor of cytokine signalling proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation.
Function:
May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Similarity:
Contains 7 ANK repeats.
Contains 1 SOCS box domain.
SWISS:
Q8WXI3
Gene ID:
136371
Database links:
Entrez Gene: 136371 Human
Omim: 615054 Human
SwissProt: Q8WXI3 Human
Unigene: 647081 Human
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Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASB10) Polyclonal Antibody, Unconjugated (SL4653R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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