Rabbit Anti-MSR1 antibody | Sunlong Medical

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CD204; SP-A; CD204 antigen; Macrophage acetylated LDL receptor I and II; Macrophage scavenger receptor 1; Macrophage Scavenger Receptor I; Macrophage scavenger receptor type III; Macrophage scavenger receptor types I and II; Msr 1; MSR1; MSRE_HUMAN; phSR1

Cat:

SL6763R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human MSR1/CD204:211-320/451<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugLane 3: Liver (Mouse) Lysate at 40 ugLane 4: Placenta (Mouse) Lysate at 40 ugLane 5: Cerebrum (Mouse) Lysate at 40 ugLane 6: Raw264.7 (Mouse) Cell Lysate at 30 ugLane 7: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 8: Liver (Rat) Lysate at 40 ugLane 9: Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-MSR1 (SL6763R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50/39 kDObserved band size: 50 kDSample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti-MSR1 (SL6763R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: Bone (Mouse) Lysate at 40 ugPrimary: Anti-MSR1 (SL6763R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDTissue/cell: mouse testis tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated(SL6763R-FITC) 1:100, 60 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiTissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated(SL6763R-FITC) 1:100, 60 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiBlank control(Blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-MSR1 antibody(SL6763R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]

Function:
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL.

Subunit:
Homotrimer.

Subcellular Location:
Membrane; Single-pass type II membrane protein.

Tissue Specificity:
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages.

DISEASE:
Defects in MSR1 may be a cause of prostate cancer (PC)[MIM:171367]. A malignancy originating in tissues of the prostate.Most prostate cancers are adenocarcinomas that develop in the aciniof the prostatic ducts. Other rare histopathologic types ofprostate cancer that occur in approximately 5% of patients includesmall cell carcinoma, mucinous carcinoma, prostatic ductalcarcinoma, transitional cell carcinoma, squamous cell carcinoma,basal cell carcinoma, adenoid cystic carcinoma (basaloid),signet-ring cell carcinoma and neuroendocrine carcinoma. Note=MSR1variants may play a role in susceptibility to prostate cancer. MSR1variants have been found in individuals with prostate cancer andco-segregate with the disease in some families.
Defects in MSR1 may be a cause of Barrett esophagus (BE)[MIM:614266]. A condition characterized by a metaplastic change inwhich normal esophageal squamous epithelium is replaced by acolumnar and intestinal-type epithelium. Patients with Barrettesophagus have an increased risk of esophageal adenocarcinoma. Themain cause of Barrett esophagus is gastroesophageal reflux. Theretrograde movement of acid and bile salts from the stomach intothe esophagus causes prolonged injury to the esophageal epitheliumand induces chronic esophagitis, which in turn is believed totrigger the pathologic changes. Note=Genetic variants in MSR1 havebeen found in individuals with Barrett esophagus and are thought tocontribute to disease susceptibility.

Similarity:
Contains 1 collagen-like domain.
Contains 1 SRCR domain.

SWISS:
P21757

Gene ID:
4481

Database links:

Entrez Gene: 281311 Cow

Entrez Gene: 4481 Human

Entrez Gene: 498638 Rat

Omim: 153622 Human

SwissProt: P21758 Cow

SwissProt: P21757 Human

Unigene: 147635 Human

Unigene: 231068 Rat

Picture

Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-MSR1 (SL6763R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-MSR1 (SL6763R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Tissue/cell: mouse testis tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated(SL6763R-FITC) 1:100, 60 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated(SL6763R-FITC) 1:100, 60 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Blank control(Blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-MSR1 antibody(SL6763R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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