Rabbit Anti-Biglycan antibody

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BGN; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A.

Cat:

SL7552R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Biglycan:101-200/368

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Bone (mouse) Lysate at 40 ugPrimary: Anti- Biglycan (SL7552R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37kDObserved band size: 52 kDSample: Colon (Rat) Lysate at 40 ugEmbryo (Mouse) Lysate at 40 ugPrimary: Anti-Biglycan (SL7552R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 37 kDObserved band size: 52 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (SL7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(SL7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human endothelial cells;4% Paraformaldehyde-fixed; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(SL7552R) 1:50, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Rhodamine B conjugated (SL0295G-RBITC) used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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May be involved in collagen fiber assembly.
Tissue specificity; Found in several connective tissues, especially in articular cartilages.

Function:
May be involved in collagen fiber assembly (By similarity).

Subunit:
Homodimer. Forms a ternary complex with MFAP2 and ELN

Subcellular Location:
Secreted, extracellular space, extracellular.

Tissue Specificity:
Found in several connective tissues, especially in articular cartilages.

Post-translational modifications:
The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity).

Similarity:
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.

SWISS:
P21810

Gene ID:
633

Database links:

Entrez Gene: 633 Human

Entrez Gene: 12111 Mouse

Entrez Gene: 25181 Rat

Omim: 301870 Human

SwissProt: P21810 Human

SwissProt: P28653 Mouse

SwissProt: P47853 Rat

Unigene: 821 Human

Unigene: 2608 Mouse

Unigene: 783 Rat

Picture

Sample:
Colon (Rat) Lysate at 40 ug
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-Biglycan (SL7552R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 37 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (SL7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(SL7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human endothelial cells;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(SL7552R) 1:50, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Rhodamine B conjugated (SL0295G-RBITC) used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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