Rabbit Anti-phospho-PI3 Kinase p110 beta (Ser1070) antibody

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PI3-kinase p110 subunit beta; p-PI3Kβ(Ser1070); PI 3-kinase C2β; p110 BETA; p110Beta; Phosphatidylinositol 3 kinase catalytic beta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4 5 bisphos

Cat:

SL6417R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Guinea Pig,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human PI3 Kinase p110 beta around the phosphorylation site of Ser1070:YR(p-S)

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human K562 cell lysates Primary: Anti-phospho-PI3 Kinase p110 beta (Ser1070) (SL6417R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 110-123 kDObserved band size: 110 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (SL6417R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (SL6417R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (SL6417R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (SL6417R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (SL6417R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Phosphoinositide 3-kinases (PI 3-Ks) phosphorylate the 3’- OH position of the inositol ring of inositol lipids. They act as participants in signaling pathways that regulate cell growth by virtue of their activation in response to various mitogenic stimuli. PI 3-Ks are composed of a catalytic subunit, such as PI 3-kinase C2∫ (PIK3CB) and an adaptor subunit. PI 3-kinase C2∫ , also known as p110-∫, is a 1,070 amino acid protein that shares 42% identity with p110 of cow origin. It is expressed in several human and rodent cell lines. Studies predict that PI 3-kinase C2∫ has a role in modulating the formation and stability of å2B (ITGA2B)/∫3 (ITGB3) Integrin adhesion bonds, which are essential in shear force-induced platelet activation. Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.

Subunit:
Heterodimer of a catalytic subunit PIK3CB and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interaction with PIK3R2 is required for nuclear localization and nuclear export. Part of a complex with PIK3R1 and PTEN. Binding to PTEN may antagonize the lipid kinase activity under normal growth conditions. Part of a complex involved in autophagosome formation composed of PIK3C3 and PIK3R4 (By similarity). Interacts with BECN1, ATG14 and RAB5A.

Subcellular Location:
Cytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export.

Tissue Specificity:
Expressed ubiquitously.

Similarity:
Belongs to the PI3/PI4-kinase family.
Contains 1 C2 PI3K-type domain.
Contains 1 PI3K-ABD domain.
Contains 1 PI3K-RBD domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.

SWISS:
P42338

Gene ID:
5291

Database links:

Entrez Gene: 5291 Human

Entrez Gene: 74769 Mouse

Entrez Gene: 85243 Rat

Omim: 602925 Human

SwissProt: P42338 Human

SwissProt: Q24JU2 Human

SwissProt: Q3U4Q1 Mouse

SwissProt: Q8BTI9 Mouse

SwissProt: Q9Z1L0 Rat

Unigene: 239818 Human

Unigene: 213128 Mouse

Unigene: 44268 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (SL6417R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (SL6417R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (SL6417R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (SL6417R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Specific References (19) | SL6417R has been referenced in 19 publications.

[IF=3.514] Li B et al. Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264. 7 macrophages.Cytokine. 2019 Jul;119:168-174. WB ; Mouse.

PubMed:30925325

[IF=4.034] Siyuan Liu. et al. β-Hydroxybutyrate impairs the release of bovine neutrophil extracellular traps through inhibiting phosphoinositide 3-kinase–mediated nicotinamide adenine dinucleotide phosphate oxidase reactive oxygen species production. J Dairy Sci. 2022 Feb;: WB ; Cow.

PubMed:35123783

[IF=5.246] Yin Zhuang. et al. Exosomes Secreted by Nucleus Pulposus Stem Cells Derived From Degenerative Intervertebral Disc Exacerbate Annulus Fibrosus Cell Degradation via Let-7b-5p. Front Mol Biosci. 2021; 8: 766115 WB ; Human.

PubMed:3512368

[IF=11.508] Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12 WB ; Mouse.

PubMed:33431863

[IF=2.276] Jianwen Longet al. Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3K/Akt/mTOR Signaling Pathway. Biomed Res Int . 2020 Jul 17;2020:5149417. WB ; Human.

PubMed:32733943

[IF=1.487] Shujuan Liuet al. Pou2F3 silencing enhanced the proliferation of mammary epithelial cells in dairy Goat via PI3K/AKT/mTOR signaling pathway. WB ; Goats.

PubMed:32730101

[IF=5.546] Liu X et al. miR‐34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ‐8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways. J Cell Physiol. 2020 Dec;235(12):10051-10067. WB ; Goat.

PubMed:32474960

[IF=4.831] Chen Y et al. Epothilone B prevents lipopolysaccharide-induced inflammatory osteolysis through suppressing osteoclastogenesis via STAT3 signaling pathway. Aging (Albany NY). 2020 Jun 11;12(12):11698-11716. WB ; Mouse.

PubMed:32527985

PubMed:32474960

[IF=1.98] Yang G et al. Ginsenoside Rb1 Prevents Dysfunction of Endothelial Cells by Suppressing Inflammatory Response and Apoptosis in the High-Fat Diet Plus Balloon Catheter-Injured Rabbit Model via the G Protein-Coupled Estrogen Receptor-Mediated Phosphatidylinositol 3-Kinases (PI3K)/Akt Pathway. Med Sci Monit. 2019 Oct 3;25:7407-7417. WB ; Rabbit.

PubMed:31609302

[IF=3.448] Jin Y et al. miR‐496 remedies hypoxia reoxygenation–induced H9c2 cardiomyocyte apoptosis via Hook3‐targeted PI3k/Akt/mTOR signaling pathway activation. J Cell Biochem. 2019 Aug 22. WB ; Rat&Human.

PubMed:31436348

[IF=3.17] Yang RP et al. Pharmacological actions of neferine in the modulation of human platelet function. Eur J Pharmacol. 2019 Aug 21:172626. WB ; Human.

PubMed:31445013

[IF=4.868] Wang Z et al. Interaction between Endothelin-1 and Left Stellate Ganglion Activation: A Potential Mechanism of Malignant Ventricular Arrhythmia during Myocardial Ischemia. Oxid Med Cell Longev. 2019 May 12;2019:6508328. WB ; Dog.

PubMed:31214281

[IF=1.41] Wei R et al. Astragaloside Ⅳ combating liver cirrhosis through the PI3K/Akt/mTOR signaling pathway. EXPERIMENTAL AND THERAPEUTIC MEDICINE.2019 17: 393-397. WB ; Rat.

PubMed:10.3892/etm.2018.6966

[IF=5.253] Liu,et al.Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.(2018) Angiogenesis. 21:237-249. WB ; Human.

PubMed:29299781

[IF=2.11] Qiao, Jingwen, et al. "Involvement of Akt/GSK3β/CREB signaling pathway on chronic omethoate induced depressive-like behavior and improvement effects of combined lithium chloride and astaxanthin treatment." Neuroscience Letters (2017). WB ; Mouse.

PubMed:28366776

[IF=0.23] Li, Zhao, et al. "The aqueous extract of Curcuma wenyujin rescues learning and memory deficits through PI3k/Akt/GSK-3β pathway in Aβ-induced AD mice." Biomedical Research 28.17 (2017): 7438-7442. WB ; Mouse.

PubMed:BiomedicalResearch(0970-938X).28(17):7438-7442.

[IF=3.15] Han Chen. et al. ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-κB signaling pathway. Bmc Cancer. 2020 Dec;20(1):1-10 WB ; Human.

PubMed:32891122

[IF=5.201] Zhang Y et al. A Regulatory Circuit Orchestrated by Novel-miR-3176 Modulates Mammary Gland Development. Front Cell Dev Biol. 2020 Jun 16;8:383. WB ; Mouse&Goat.

PubMed:32656203