Rabbit Anti-LRCH1 antibody | Sunlong Medical

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Calponin homology domain-containing protein 1; CHDC1; KIAA1016; Leucine-rich repeat and calponin homology domain-containing protein 1 antibody Lrch1; LRCH1_HUMAN; Neuronal protein 81; NP81.

Cat:

SL9327R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human LRCH1:221-320/728

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-LRCH1 (SL9327R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 81 kDSample: U251 Cell (Human) Lysate at 30 ugPrimary: Anti- LRCH1 (Bs- 9327R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 81 kDSample: Cerebellum (Mouse) Lysate at 40 ugPrimary: Anti- LRCH1 (Bs- 9327R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 81 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRCH1) Polyclonal Antibody, Unconjugated (SL9327R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRCH1) Polyclonal Antibody, Unconjugated (SL9327R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Members of the leucine-rich repeat family include LRCH1, LRCH2, LRCH3 and LRCH4. All family members contain one calponin-homology domain and nine leucine-rich repeats. The gene encoding LRCH1, also known as Neuronal protein 81 (NP81), has been suggested to be a possible gene locus for susceptibility to osetoarthritis, especially in women, though studies have shown conflicting results. LRCH1 is a 728 amino acid protein that is expressed at highest levels in cerebellum, with lower levels of expression in spinal cord, ovary, kidney and amygdala. In pigs, the gene encoding LRCH1 is one of 22 genes that is associated with leg and body conformation traits that affect productivity and health. There are two isoforms of LRCH1 that are expressed as a result of alternative splicing events.

Similarity:
Contains 1 CH (calponin-homology) domain.
Contains 9 LRR (leucine-rich) repeats.

SWISS:
Q9Y2L9

Gene ID:
23143

Database links:

Entrez Gene: 23143 Human

Omim: 610368 Human

SwissProt: Q9Y2L9 Human

Unigene: 507971 Human

Picture

Sample:
U251 Cell (Human) Lysate at 30 ug
Primary: Anti- LRCH1 (Bs- 9327R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD

Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- LRCH1 (Bs- 9327R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRCH1) Polyclonal Antibody, Unconjugated (SL9327R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRCH1) Polyclonal Antibody, Unconjugated (SL9327R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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