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Home > Product > Antibody > Rabbit Anti-CD4 antibody
CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4 ; T cell antigen T4/LEU3; T cell differentiation antigen L3T
Cat:
SL4817R
Species Reactivity：
Human,Mouse,Rat,
Immunogen：
KLH conjugated synthetic peptide derived from human CD4:301-400/458<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Spleen (Rat) Lysate at 40 ugPrimary: Anti-CD4 (SL4817R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kDSample: Spleen (Rat) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugLymph node (Rat) Lysate at 40 ugLymph node (Mouse) Lysate at 40 ugThymus (Rat) Lysate at 40 ugThymus (Mouse) Lysate at 40 ugSmall intestine (Rat) Lysate at 40 ugSmall intestine (Mouse) Lysate at 40 ugLarge intestine (Rat) Lysate at 40 ugLarge intestine (Mouse) Lysate at 40 ugHL60 (Human) Cell Lysate at 30 ugJurkat (Human) Cell Lysate at 30 ugPrimary: Anti-CD4 (SL4817R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 52 kDSample: Lymphoid (Rat) Lysate at 40 ug Primary: Anti-CD4 (SL4817R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kDSample: Thymus (Mouse) Lysate at 40 ugPrimary: Anti-CD4 (SL4817R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kDSample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-CD4 (SL4817R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kDSample: HL60 Cell(human) Lysate at 30 ugPrimary: Anti-CD4 (SL4817R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kD
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Datasheet:


This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].

Function:
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Subunit:
Associates with LCK. Binds to HISLV1 gp120 and to P4HB/PDI and upon HISLV1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HISLV1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HISLV1 gp120.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HISLV1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HISLV1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

Post-translational modifications:
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Similarity:
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
P01730

Gene ID:
920

Database links:

Entrez Gene: 920 Human

Entrez Gene: 12504 Mouse

Omim: 186940 Human

SwissProt: P06332 Mouse

SwissProt: P01730 Human

Unigene: 631659 Human

Unigene: 2209 Mouse



CD4分子是存在于大多数辅助/诱导T细胞表面的59kDa的糖蛋白。正常淋巴组织中CD4的表达数量多于CD8，此抗体主要用于标记辅助/诱导T细胞，与CD8单抗联合使用对外周血淋巴细胞分型。 CD4抗原是HLA-II类分子和人类免疫缺陷病毒(HIV)-爱滋病的受体，在35-50%外周血淋巴细胞-辅助和诱导T细胞(Th/Ti)和70-80%人胸腺细胞上表达，在人的单核细胞表面也有低密度的表达。 CD4抗原有膜结合型和可溶性两种形式。Th/Ti可辅助Ig产生和T细胞毒T细胞的作用。 Picture

Sample:
Spleen (Rat) Lysate at 40 ug
Primary: Anti-CD4 (SL4817R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Sample:
Spleen (Rat) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Rat) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Thymus (Rat) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Small intestine (Rat) Lysate at 40 ug
Small intestine (Mouse) Lysate at 40 ug
Large intestine (Rat) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
HL60 (Human) Cell Lysate at 30 ug
Jurkat (Human) Cell Lysate at 30 ug
Primary: Anti-CD4 (SL4817R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
Sample:
Lymphoid (Rat) Lysate at 40 ug
Primary: Anti-CD4 (SL4817R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-CD4 (SL4817R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD4 (SL4817R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Sample:
HL60 Cell(human) Lysate at 30 ug
Primary: Anti-CD4 (SL4817R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
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