C11orf9 (chromosome 11 open reading frame 9), also known as MRF (myelin gene regulatory factor), Ndt80, KIAA0954 or MGC10781, is a 1,151 amino acid single-pass membrane protein that localizes to nucleus. A member of the MRF family, C11orf9 is expressed in the ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. C11orf9 is weakly expressed in cerebellum and retina. C11orf9 contains one NDT80 DNA-binding domain and acts as a transcription factor that is required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. C11orf9 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 11, which consists of approximately 135 million base pairs and 1,400 genes. Chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome.
Function:
Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.
Subunit:
Belongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
Subcellular Location:
Membrane. Nucleus.
Tissue Specificity:
Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
Similarity:
Belongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
SWISS:
Q9Y2G1
Gene ID:
745
Database links:
Entrez Gene: 225908 Mouse
SwissProt: Q3UR85 Mouse
Unigene: 329716 Mouse
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Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-MRF/C11orf9 (SL11191R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U2OS cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated(SL11191R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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