Rabbit Anti-Phospho-EIF2S1 (Ser51) antibody

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EIF2S1 (phospho S51); EIF2S1 (phospho-Ser51); EIF2A; CDA 02; CDA02; eIF 2 alpha; EIF 2; EIF 2A; EIF-2alpha; EIF2; EIF2alpha; eIF2S1; Eukaryotic Translation Initiation Factor 2 alpha; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukary

Cat:

SL4842R

Species Reactivity：

Human,Mouse,Rat,(predicted: Cow,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human eIF2 alpha around the phosphorylation site of Ser51:EL(p-S)RR

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-eIF2 alpha(Ser51) (SL4842R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 34 kDSample: Ovary (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-eIF2 alpha(Ser51) (SL4842R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 34 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-eIF2 alpha(Ser51)) Polyclonal Antibody, Unconjugated (SL4842R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-eIF2 alpha(Ser51)) Polyclonal Antibody, Unconjugated (SL4842R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Blank control(blue): U-87MG(fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Phospho-eIF2 alpha(Ser51) antibody(SL4842R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010].

Function:
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Subunit:
Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation. Interacts with ABCF1 isoform 2. Associates with ribosomes.

Subcellular Location:
Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules.

Post-translational modifications:
Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus A, eIF2S1 phosphorylation state is modulated.

Similarity:
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.

SWISS:
P05198

Gene ID:
1965

Database links:

Entrez Gene: 1965 Human

Entrez Gene: 13665 Mouse

Entrez Gene: 54318 Rat

Omim: 603907 Human

SwissProt: P05198 Human

SwissProt: Q6ZWX6 Mouse

SwissProt: P68101 Rat

Unigene: 151777 Human

Unigene: 196220 Mouse

Unigene: 1488 Rat

Picture

Sample: Ovary (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-eIF2 alpha(Ser51) (SL4842R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-eIF2 alpha(Ser51)) Polyclonal Antibody, Unconjugated (SL4842R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-eIF2 alpha(Ser51)) Polyclonal Antibody, Unconjugated (SL4842R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Blank control(blue): U-87MG(fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Phospho-eIF2 alpha(Ser51) antibody(SL4842R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Product Feedback Wall

Specific References (10) | SL4842R has been referenced in 10 publications.

[IF=4.872] Yu Wang. et al. Zinc offers splenic protection through suppressing PERK/IRE1-driven apoptosis pathway in common carp (Cyprinus carpio) under arsenic stress. Ecotox Environ Safe. 2021 Jan;208:111473 WB ; Fish.

PubMed:33068983

[IF=3.184] Bingwei Yang et al. Polychlorinated Biphenyl Quinone Promotes Atherosclerosis through Lipid Accumulation and Endoplasmic Reticulum Stress via CD36. Chem Res Toxicol. 2020 Jun 15;33(6):1497-1507. WB ; Mouse.

PubMed:32434321

[IF=14.024] Berger,et al.Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment.(2018) Neuron. 97:555-570.e6. IF(ICC) ; Mouse.

PubMed:29395909

[IF=3.858] Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167. WB ; Human.

PubMed:29306025

[IF=11.329] Yao et al. A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure. (2017) Nat.Commu. 8:133 WB ; Human.

PubMed:28743963

[IF=3.687] Cha et al. miR-211 Plays a Critical Role in Cnidium officinale Makino Extract-Induced, ROS/ER Stress-Mediated Apoptosis in U937 and U266 Cells. (2018) Int.J.Mol.Sci.Mar 15;19(3). WB ;

PubMed:29543750

[IF=3.224] Yu Wang. et al. Co-administration of zinc for treating and preventing arsenism in common carp Cyprinus carpio: An alternative to avoid physiological and cellular damages. Aquaculture. 2021 Jan;531:735965 WB ; Fish.

PubMed:10.1016/j.aquaculture.2020.735965

[IF=38.637] Qiyi Zhao. et al. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output. Cell. 2020 Oct;183:76 WB ; Humann.

PubMed:32931733

[IF=2.971] Wenfeng Gouet al. Ursolic acid derivative UA232 evokes apoptosis of lung cancer cells induced by endoplasmic reticulum stress. Pharm Biol . 2020 Dec;58(1):707-715. WB ; Human.

PubMed:32726164

[IF=2.33] He, Yihuai, et al. "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." Molecular and Cellular Biochemistry (2014): 1-8. WB ; Human.

PubMed:24390087