Rabbit Anti-PDE7B antibody | Sunlong Medical

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PDE 7B; PDE7B protein; Phosphodiesterase 7B; Phosphodiesterase7B; RNPDE7B; Rolipram insensitive phosphodiesterase type 7; bA472E5.1; cAMP specific 3 5 cyclic phosphodiesterase 7B; cAMP specific 3'5' cyclic phosphodiesterase 7B; High affinity cAMP specific

Cat:

SL11576R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human PDE7B:281-360/450

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-PDE7B (SL11576R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 52 kDSample: Brain (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugPrimary: Anti-PDE7B (SL11576R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 52 kDObserved band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (PDE7B) Polyclonal Antibody, Unconjugated (SL11576R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. raformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (PDE7B) Polyclonal Antibody, Unconjugated (SL11576R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control(blue): RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- PDE7B antibody(SL11576R), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Phosphodiesterases (PDE, also designated cyclic nucleotide phosphodiesterase) are important for the downregulation of the intracellular level of the second messenger cyclic adenosine monophosphate (cAMP) by hydrolyzing cAMP to 5'AMP. Phosphodiesterase type 3 isoforms, PDE3A and 3B, are expressed primarily in cardiovascular tissue and adipose tissue, respectively. PDE3A, is found in myocardium and platelets and PDE3B is found in lymphocytes. The PDE7A1 (HCP1) isozyme and the PDE7A2 proteins, alternate splice products of PDE7A, are highly expressed in skeletal muscle. PDE7B is most highly expressed in pancreas. The PDE family contains proteins that serve tissue-specific roles in regulation of lipolysis, glycogenolysis, myocardial contractility, and smooth muscle relaxation.

Function:
PDE7B is a cAMP specific phosphodiesterase. The cAMP-specific phosphodiesterase type-7 (PDE7) family is comprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE7B has three splice variants (PDE7B1 = 446 amino acid; PDE7B2 =359 amino acids and PDE7B3 = 459 amino acids. Both PDE7B2 ad PDE7B3 posses unique N-terminal sequences. PDE7B is expressed in various tissues including skeletal muscle, heart, lung and testis. PDE7B may be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.

Tissue Specificity:
Highly expressed in brain. Also expressed in heart, liver, skeletal muscle and pancreas.

Similarity:
Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.

SWISS:
Q9NP56

Gene ID:
27115

Database links:

Entrez Gene: 27115 Human

Entrez Gene: 29863 Mouse

Entrez Gene: 140929 Rat

Omim: 604645 Human

SwissProt: Q9NP56 Human

SwissProt: Q9QXQ1 Mouse

Unigene: 153615 Rat

Picture

Sample:
Brain (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-PDE7B (SL11576R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (PDE7B) Polyclonal Antibody, Unconjugated (SL11576R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

raformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (PDE7B) Polyclonal Antibody, Unconjugated (SL11576R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control(blue): RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti- PDE7B antibody(SL11576R), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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