Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- PPP2R2B (SL11749R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample: HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti- PPP2R2B/PP2A-B55 (SL11749R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD
Sample: A431 Cell (Human) Lysate at 40 ug
Primary: Anti- PPP2R2B/PP2A-B55 (SL11749R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R2B/PP2A-B55 (SL11749R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R2B,PP2A-B55) Polyclonal Antibody, Unconjugated (SL11749R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|