Rabbit Anti-TMEM158 antibody

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40 kDa BINP binding protein; 40 kDa BINP-binding protein; BBP; BINP receptor; Brain injury derived neurotrophic peptide (BINP) binding protein; Brain specific binding protein; DKFZp586E1621; HBBP; p40BBP; Ras induced senescence protein 1; Ras-induced sene

Cat:

SL11770R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human TMEM158:188-260/300

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (SL11770R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TMEM158 Polyclonal Antibody, Unconjugated(SL11770R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (SL11770R) at 1:400 overnight at 4°C, followed by a conjugated secondary (SL0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei. Blank control: Mouse spleen cells(blue). Primary Antibody: Rabbit Anti-TMEM158 antibody(SL11770R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Blank control: Mouse heart cells(blue). Primary Antibody: Rabbit Anti-TMEM158 antibody(SL11770R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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TMEM158 is a 286 amino acid protein encoded by a gene mapping to human chromosome 3. Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.

Function:
Receptor for brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide.

Subcellular Location:
Membrane.

Post-translational modifications:
N-glycosylated.

Similarity:
Belongs to the TMEM158 family.

SWISS:
Q8WZ71

Gene ID:
25907

Database links:

Entrez Gene: 717685 Cow

Entrez Gene: 25907 Human

Entrez Gene: 72309 Mouse

Entrez Gene: 117582 Rat

SwissProt: A2VDX9 Cow

SwissProt: Q8WZ71 Human

SwissProt: Q6F5E0 Mouse

SwissProt: Q91XV7 Rat

Unigene: 740403 Human

Unigene: 8569 Mouse

Unigene: 4294 Rat

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TMEM158 Polyclonal Antibody, Unconjugated(SL11770R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (SL11770R) at 1:400 overnight at 4°C, followed by a conjugated secondary (SL0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.

Blank control: Mouse spleen cells(blue).
Primary Antibody: Rabbit Anti-TMEM158 antibody(SL11770R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Blank control: Mouse heart cells(blue).
Primary Antibody: Rabbit Anti-TMEM158 antibody(SL11770R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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