Rabbit Anti-ALDH1A1 antibody

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cytosolic; Acetaldehyde dehydrogenase 1; AHD2; Ahd-2; AL1A1_HUMAN; ALDC; Aldehyde dehydrogenase 1 family member A1; Aldehyde dehydrogenase 1 soluble; Aldehyde dehydrogenase 1A1; Aldehyde Dehydrogenase; Aldehyde dehydrogenase cytosolic; Aldehyde dehydrogen

Cat:

SL10162R

Species Reactivity：

Human,Mouse,(predicted: Rat,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ALDH1:201-300/501

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Placenta (Mouse) Lysate at 40 ugPrimary: Anti-ALDH1 (SL10162R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample: Liver (mouse) Lysate at 40 ugPrimary: Anti- ALDH1 (SL10162R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample:A549 Cell (Human) Lysate at 40 ugHepG2 Cell (Human) Lysate at 40 ugPrimary: Anti-ALDH1(SL10162R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54kDObserved band size: 54kDParaformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (SL10162R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (SL10162R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Function:
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.

Subunit:
Homodimer.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed in the liver, lung, and testis. Apparently not expressed at detectable levels in kidney, stomach, ovary, heart, and brain.

Similarity:
Belongs to the aldehyde dehydrogenase family.

SWISS:
P00352

Gene ID:
216

Database links:

Entrez Gene: 216 Human

Entrez Gene: 11668 Mouse

Entrez Gene: 24188 Rat

Entrez Gene: 443343 Sheep

Omim: 100128 Human

SwissProt: P00352 Human

SwissProt: P24549 Mouse

SwissProt: P51647 Rat

SwissProt: P51977 Sheep

Unigene: 76392 Human

Unigene: 250866 Mouse

Unigene: 6132 Rat

Picture

Sample: Liver (mouse) Lysate at 40 ug
Primary: Anti- ALDH1 (SL10162R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample:
A549 Cell (Human) Lysate at 40 ug
HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-ALDH1(SL10162R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 54kD

Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (SL10162R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (SL10162R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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