Rabbit Anti-FKBP10 antibody

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65 kDa FK506 binding protein; FK506 binding protein 10 65 kDa; FK506 binding protein 10; FKBP 10; FKBP 65; hFKBP 65; Immunophilin FKBP65; Peptidyl prolyl cis trans isomerase. PPIase; Rotamase; FKB10_HUMAN.

Cat:

SL13175R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human FKBP10:31-130/582

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:293T Cell(Human)Lysate at 40 ugPrimary: Anti-FKBP10(SL13175R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 61kDObserved band size: 61kDSample: Uterus (Mouse) Lysate at 40 ugPrimary: Anti-FKBP10(SL13175R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 61 kDObserved band size: 76 kDSample: MCF-7(human) Cell Lysate at 40 ugPrimary: Anti-FKBP10(SL13175R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 61 kDObserved band size: 61 kDParaformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (SL13175R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (SL13175R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases that bind to and mediate the effects of immunosuppressive drugs, such as cyclosporin, FK506 and rapamycin. Immunophilins have also been implicated in protein folding and trafficking within the endoplasmic reticulum (ER). FKBP10 (FK506-binding protein 10), also known as peptidyl-prolyl cis-trans isomerase, PPIase, Rotamase, 65kDa FK506-binding protein or FKBP65, is a 582 amino acid immunophilin localized to the ER lumen and found in many tissues including heart, spleen, brain, testis and lung. FKBP10 contains two EF-hand calcium-binding domains and four PPIase FKBP-type domains, suggesting an enzymatic role in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP10 also acts as a receptor for the immunosuppressants FK506 and rapamycin, which inhibit FKBP10 activity. FKBP10 is thought to interact with the Raf-1/HSP 90 heterocomplex during signal transduction processes, and may associate with elastin during elastin protein folding and transport. With a valine-24 addition to human FKBP10, human and mouse FKBP10 are almost identical.

Function:
FKBP10 (FK506 binding protein 10) belongs to the FKBP type peptidyl-prolyl cis/trans isomerase (PPIase) family. PPIases accelerate the folding of proteins during protein synthesis, acting as molecular chaperones. FKBP10 is located in the endoplasmic reticulum (ER) and is thought to play a role in the folding and trafficking of secretory proteins.

Subcellular Location:
Endoplasmic reticulum.

Post-translational modifications:
Glycosylated and phosphorylated.

DISEASE:
Defects in FKBP10 are the cause of osteogenesis imperfecta type 6 (OI6) [MIM:610968]. OI6 is a moderate to severe, autosomal recessive form of osteogenesis imperfecta characterized by increased serum alkaline phosphatase levels and bone histology exhibiting a fish scale-like lamellar pattern. Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.

Similarity:
Contains 2 EF-hand domains.
Contains 4 PPIase FKBP-type domains.

SWISS:
Q96AY3

Gene ID:
60681

Database links:

Entrez Gene: 60681 Human

Omim: 607063 Human

SwissProt: Q96AY3 Human

Unigene: 463035 Human

Picture

Sample: Uterus (Mouse) Lysate at 40 ug
Primary: Anti-FKBP10(SL13175R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 76 kD

Sample: MCF-7(human) Cell Lysate at 40 ug
Primary: Anti-FKBP10(SL13175R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (SL13175R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (SL13175R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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