Rabbit Anti-nNOS antibody | Sunlong Medical

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NOS1_HUMAN; Nitric oxide synthase, brain; NOS1; EC:1.14.13.39; Constitutive NOS; N NOS; N-NOS; NC-NOS; NOS type I; Neuronal NOS (N-NOS; nNOS); Peptidyl-cysteine S-nitrosylase NOS1; bNOS; neuronal Nitric Oxide Synthase;

Cat:

SL10197R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human nNos/NOS-1:601-700/1434

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti- nNOS (SL10197R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130 kDObserved band size: 150 kDSample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-nNOS (SL10197R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130 kDObserved band size: 130 kDSample: Hela(Human) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugA549(Human) Cell Lysate at 30 ugRAW246.7 (Mouse) Cell Lysate at 30 ugPrimary: Anti-nNOS (SL10197R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130 kDObserved band size: 130 kD Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (nNOS) Polyclonal Antibody, Unconjugated (SL10197R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): Mouse brain (fixed with 2% paraformaldehyde for 10 min at room temperature, and then stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-nNOS antibody (SL10197R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011].

Function:
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Subunit:
Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with HTR4. Interacts with VAC14 (By similarity). Interacts with SLC6A4.

Subcellular Location:
Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).

Tissue Specificity:
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Post-translational modifications:
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).

Similarity:
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain.

SWISS:
P29475

Gene ID:
4842

Database links:

Entrez Gene: 4842 Human

Omim: 163731 Human

SwissProt: P29475 Human

Unigene: 654410 Human

Unigene: 684465 Human

Unigene: 684466 Human

Unigene: 684467 Human

一氧化氮合成酶-1催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS1）、诱导型一氧化氮合成的酶（iNOS or NOS2）、内皮型一氧化氮合成的酶（eNOS or NOS3）。此抗体识别分子量为150kDa的神经型一氧化氮合成的酶。 Picture

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-nNOS (SL10197R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
RAW246.7 (Mouse) Cell Lysate at 30 ug
Primary: Anti-nNOS (SL10197R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (nNOS) Polyclonal Antibody, Unconjugated (SL10197R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control (blue line): Mouse brain (fixed with 2% paraformaldehyde for 10 min at room temperature, and then stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-nNOS antibody (SL10197R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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