Rabbit Anti-phospho-CD130/gp130 (Ser782) antibody

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gp130 (phospho S782); gp130 (phospho Ser782); p-gp130 (Ser782); Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS;

Cat:

SL10122R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser782:SE(p-S)TQ

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T (human)cell Lysate at 40 ugHepG2 (human)cell Lysate at 40 ugPrimary: Anti- p-gp130 (Ser782) (SL10122R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 113kDObserved band size: 123 kDSample: Raji (human)cell Lysate at 40 ugPrimary: Anti- p-gp130 (Ser782) (SL10122R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 113kDObserved band size: 123 kDSample: Lane 1: Human HepG2 cell Lysates Lane 2: Human HUVEC cell Lysates Primary: Anti-phospho-CD130gp130 (Ser782) (SL10122R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130kDaObserved band size: 130kDaSample: Lane 1: HepG2 (Human) Cell Lysate at 30 ugLane 2: Hela (Human) Cell Lysate at 30 ugLane 3: A549 (Human) Cell Lysate at 30 ugLane 4: U87MG (Human) Cell Lysate at 30 ugPrimary: Anti-phospho-CD130/gp130 (Ser782) (SL10122R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130 kDObserved band size: 140 kDParaformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (SL10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (SL10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.phosphopeptidenon phosphopeptide

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Function:
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.

Subunit:
Interacts with INPP5D/SHIP1. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Homodimer. The homodimer binds two molecules of herpes virus IL6. Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Tissue Specificity:
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.

Post-translational modifications:
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated.

Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

SWISS:
P40189

Gene ID:
3572

Database links:

Entrez Gene: 3572 Human

Entrez Gene: 16195 Mouse

Entrez Gene: 25205 Rat

Omim: 600694 Human

SwissProt: P40189 Human

SwissProt: Q00560 Mouse

SwissProt: P40190 Rat

Unigene: 56482 Human

Unigene: 706627 Human

Unigene: 4364 Mouse

Unigene: 12138 Rat

Picture

Sample: Raji (human)cell Lysate at 40 ug
Primary: Anti- p-gp130 (Ser782) (SL10122R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113kD
Observed band size: 123 kD

Sample:
Lane 1: Human HepG2 cell Lysates
Lane 2: Human HUVEC cell Lysates
Primary: Anti-phospho-CD130gp130 (Ser782) (SL10122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130kDa
Observed band size: 130kDa

Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Lane 4: U87MG (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-CD130/gp130 (Ser782) (SL10122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 140 kD

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (SL10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (SL10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

phosphopeptide non phosphopeptide

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