Rabbit Anti-GPR91 antibody | Sunlong Medical

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G protein coupled receptor 91; G-protein coupled receptor 91; GPR 91; P2Y purinoceptor 1;P2Y purinoceptor 1-like; Purinergic Receptor P2U2; Succinate receptor; Succinate receptor 1; SUCN R1; SUCNR 1; SUCNR1; SUCR1_HUMAN.

Cat:

SL15392R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthetic peptide derived from human GPR91:131-334/334<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2 Cell (Human) Lysate at 40 ugPrimary: Anti-GPR91(SL15392R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39kDObserved band size: 39kDProtein: 1.kidney lyates(mo);2.liver lyates(mo);Primary: Rabbit Anti-GPR91 (SL15392R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:39 kDObserved band size:49 kDWestern blot analysis of extracts from Liver tissue(mo) using GPR91 Antibody.Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(SL15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(SL15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a G-protein-coupled receptor for succinate, an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development, and it has a potential role in renovascular hypertension which has known correlations to renal failure, diabetes and atherosclerosis. [provided by RefSeq, Oct 2009]

Function:
Receptor for succinate.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed specifically in kidney.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
Q9BXA5

Gene ID:
56670

Database links:

Entrez Gene: 56670 Human

Entrez Gene: 84112 Mouse

Entrez Gene: 408199 Rat

Omim: 606381 Human

SwissProt: Q9BXA5 Human

SwissProt: Q99MT6 Mouse

SwissProt: Q6IYF9 Rat

Unigene: 279575 Human

Unigene: 125110 Mouse

Unigene: 124512 Rat

Picture

Protein: 1.kidney lyates(mo);2.liver lyates(mo);
Primary: Rabbit Anti-GPR91 (SL15392R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:39 kD Observed band size:49 kD

Western blot analysis of extracts from Liver tissue(mo) using GPR91 Antibody.

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(SL15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(SL15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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