Rabbit Anti-TMEM176A antibody

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GS188; HCA112; Hepatocellular carcinoma-associated antigen 112; likley ortholog of mouse GS188; T176A_MOUSE; Tmem176a; Transmembrane protein 176A.

Cat:

SL13622R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from MOUSE TMEM176A:1-100/235

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Protein: HepG2(human) lysates at 30ug; Primary: rabbit Anti-TMEM176A (SL13622R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size:26 kDObserved band size:26 kDSample: U937(Human) Cell Lysate at 30 ugPrimary: Anti- TMEM176A (SL13622R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 25 kDProtein: mouse kidney lysates, 30ug; Primary: Anti-TMEM176A (SL13622R) at 1:300; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1:5000; ECL excitated the fluorescence; Predicted band size : 24kDObserved band size : 24kDParaformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (SL13622R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated(SL13622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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TMEM176A is a 235 amino acid multi-pass membrane protein belonging to the TMEM176 family. The gene encoding GS188 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. The deletion of a portion of the q arm of chromosome 7 is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual comfort and friendliness with strangers and an elfin appearance. Deletions of portions of the q arm of chromosome 7 are also seen in a number of myeloid disorders including cases of acute myelogenous leukemia and myelodysplasia.

Subcellular Location:
Membrane.

Similarity:
Belongs to the TMEM176 family.

SWISS:
Q96HP8

Gene ID:
55365

Database links:

Entrez Gene: 55365 Human

Omim: 610334 Human

SwissProt: Q96HP8 Human

Unigene: 647116 Human

Picture

Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- TMEM176A (SL13622R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 25 kD

Protein: mouse kidney lysates, 30ug;
Primary: Anti-TMEM176A (SL13622R) at 1:300;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1:5000;
ECL excitated the fluorescence;
Predicted band size : 24kD
Observed band size : 24kD

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (SL13622R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated(SL13622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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