EXDL1 is a 514 amino acid protein that belongs to the EXD1 family and contains one 3'-5' exonuclease domain. Existing as two alternatively spliced isoforms, the gene encoding EXDL1 maps to human chromosome 15q15.1 and mouse chromosome 2 E5. Encoding more than 700 genes, chromosome 15 is made up of approximately 106 million base pairs and is about 3% of the human genome. Angelman and Prader-Willi syndromes are associated with loss of function or deletion of genes in the 15q11-q13 region. In the case of Angelman syndrome, this loss is due to inactivity of the maternal 15q11-q13 encoded UBE3A gene in the brain by either chromosomal deletion or mutation. In cases of Prader-Willi syndrome, there is a partial or complete deletion of this region from the paternal copy of chromosome 15.
Similarity:
Belongs to the EXD1 family.
Contains 1 3'-5' exonuclease domain.
SWISS:
Q8NHP7
Gene ID:
161829
Database links:
Entrez Gene: 161829 Human
SwissProt: Q8NHP7 Human
Unigene: 307999 Human
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Sample: A549 (Human) Cell Lysate at 40 ug
Primary: Anti-EXDL1(SL14656R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 52 kD
Sample:
Raji Cell (Human) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti-EXDL1 (SL14656R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample:HT29 (Human) Cell Lysate at 40 ug
Primary: Anti-EXDL1(SL14656R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample: 293T (Human)Cell Lysate at 40 ug
Primary: Anti-EXDL1(SL14656R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXDL1) Polyclonal Antibody, Unconjugated (SL14656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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