Rabbit Anti-phospho-IRE1 (Ser724) antibody

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IRE1 (phospho S724); p-IRE1 (phospho S724); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ER to nucleus signalli

Cat:

SL16698R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724:RH(p-S)FS

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-IRE1 (Ser724) (SL16698R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 107 kDObserved band size: 112 kDSample: Panc-1(Human) Cell Lysate at 30 ugPrimary: Anti-phospho-IRE1 (Ser724) (SL16698R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 107 kDObserved band size: 107 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway.

Function:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new SLCterminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Subcellular Location:
Endoplasmic reticulum membrane.

Tissue Specificity:
Ubiquitously expressed. High levels observed in pancreatic tissue.

Post-translational modifications:
Autophosphorylated.

Similarity:
Belongs to the protein kinase superfamily.
Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.

SWISS:
O7592

Gene ID:
2081

Database links:

Entrez Gene: 10595 Human

Entrez Gene: 2081 Human

Entrez Gene: 78943 Mouse

Entrez Gene: 419613 Rat

Omim: 604033 Human

SwissProt: O7592 Human

SwissProt: Q76MJ5 Human

SwissProt: Q9EQY0 Mouse

SwissProt: Q9Z2E3 Mouse

Unigene: 133982 Human

Unigene: 592041 Human

Unigene: 700027 Human

Unigene: 20452 Mouse

Unigene: 340943 Mouse

Unigene: 226435 Rat

Picture

Sample:
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-IRE1 (Ser724) (SL16698R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 107 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Specific References (8) | SL16698R has been referenced in 8 publications.

[IF=6.543] Fan Kexia. et al. Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas. Oxid Med Cell Longev. 2022;2022:5652586 WB ; Mouse.

PubMed:35368865

[IF=5.396] Yujie Zhong. et al. Dioscin relieves diabetic nephropathy via suppressing oxidative stress, apoptosis, and improving mitochondrial quality and quantity control. Food Funct. 2022 Feb;: WB ; Rat.

PubMed:35262539

[IF=5.878] Xueying Zhang. et al. Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1α/XBP1 pathway. 2021 Dec 02 WB ; Mouse ,Human.

PubMed:34859513

[IF=6.023] Yujie Zhong. et al. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy. Food Chem Toxicol. 2022 Jan;159:112697 WB ; Rat.

PubMed:34826549

[IF=4.294] Mei Zhang. et al. Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. Neurotoxicology. 2022 Jan;88:187 WB ; Human.

PubMed:34813867

[IF=3.038] Jiajia Chenet al. Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1. Neurochem Res . 2020 Oct;45(10):2302-2311. WB ; rat.

PubMed:32681444

[IF=4.52] Zhu YP et al. Unification of Opposites between Two Antioxidant Transcription Factors Nrf1 and Nrf2 in Mediating Distinct Cellular Responses to the Endoplasmic Reticulum Stressor Tunicamycin. Antioxidants (Basel). 2019 Dec 19;9(1). pii: E4. WB ; Human.

PubMed:31861550

[IF=4.868] Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742. WB ; Mouse.

PubMed:30728884