Rabbit Anti-CRHR1 antibody | Sunlong Medical

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CRF1; Corticotropin releasing hormone receptor variant 1h;CRF-RI; CRF 1; CRF R; CRFR1; CRH R1h; CRF-R1; CRHR 1; CRHR; CRHR1f; Releasing hormone receptor 1; Seven transmembrane helix receptor; corticotropin releasing hormone receptor 1; Crhr; MGC124237; MG

Cat:

SL10248R

Species Reactivity：

Mouse,Rat,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human CRHR1:55-150/444<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: muscle (mouse) Lysate at 40 ugPrimary: Anti-CRHR1(SL10248R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 58 kDSample: kidney (mouse) Lysate at 40 ugPrimary: Anti-CRHR1(SL10248R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 58 kDSample: heart (mouse) Lysate at 40 ugPrimary: Anti-CRHR1(SL10248R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 58 kDSample: Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Cerebellum (Mouse) Lysate at 40 ugLane 3: Testis (Mouse) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: Cerebellum (Rat) Lysate at 40 ugPrimary: Anti-CRHR1 (SL10248R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50-55 kDObserved band size: 48 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated(SL10248R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingPositive control: RSC96 cellsConcebtration: 5ug/10^6 cellsIncubation conditions: Avoid light , 30 minutes on the ice.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants one of which is a non-coding read-through transcript with the neighboring gene MGC57346.

Function:
Receptor for corticotropin releasing factor (CRH). Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subunit:
Interacts (via N-terminal extracellular domain) with CRF and UCN.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.

Post-translational modifications:
SLCterminal Ser or Thr residues may be phosphorylated.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

SWISS:
P34998

Gene ID:
1394

Database links:

Entrez Gene: 1394 Human

Entrez Gene: 12921 Mouse

Entrez Gene: 58959 Rat

Omim: 122561 Human

SwissProt: P34998 Human

SwissProt: P35347 Mouse

SwissProt: P35353 Rat

Unigene: 417628 Human

Unigene: 1892 Mouse

Unigene: 10499 Rat

Picture

Sample: kidney (mouse) Lysate at 40 ug
Primary: Anti-CRHR1(SL10248R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 58 kD

Sample: heart (mouse) Lysate at 40 ug
Primary: Anti-CRHR1(SL10248R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 58 kD

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Testis (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-CRHR1 (SL10248R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kD
Observed band size: 48 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated(SL10248R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Positive control: RSC96 cells
Concebtration: 5ug/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.

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