Rabbit Anti-CLEC16A antibody

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Protein CLEC16A; C type lectin domain family 16 member A; CL16A; Gop 1; Gop1; CL16A_HUMAN； KIAA0350; MGC111457.

Cat:

SL18539R

Species Reactivity：

Human,(predicted: Mouse,Rat,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human CLEC16A

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: k562 (human)Cell Lysate at 40 ugPrimary: Anti-CLEC16A(SL18539R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 118 kDObserved band size: 118 kDSample: K562(Human) Cell Lysate at 30 ugSiha(Human) Cell Lysate at 30 ugU-2OS(Human) Cell Lysate at 30 ugU87MG(Human) Cell Lysate at 30 ugPrimary: Anti- CLEC16A (SL18539R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 118 kDObserved band size: 118 kD Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (SL18539R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(SL18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(SL18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the SLCtype lectin domain containing family. Single nucleotide polymorphisms in introns of this gene have been associated with diabetes mellitus, multiple sclerosis and rheumatoid arthritis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Subcellular Location:
Cell membrane, Cytoplasmic.

Tissue Specificity:
Almost exclusively expressed in immune cells, including dendritic cells, B-lymphocytes and natural killer cells.

DISEASE:
Insulin-dependent diabetes mellitus is significantly associated with variation within a 233-kb linkage disequilibrium block on chromosome 16p13 that includes KIAA0350. Three common non-coding variants of KIAA0350 in strong linkage disequilibrium reach genome-wide significance for association with the disease.

Similarity:
Belongs to the CLEC16A/gop-1 family.

SWISS:
Q2KHT3

Gene ID:
23274

Database links:

Entrez Gene: 23274 Human

Entrez Gene: 74374 Mouse

Omim: 611303 Human

SwissProt: Q2KHT3 Human

SwissProt: Q80U30 Mouse

Picture

Sample:
K562(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
U-2OS(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- CLEC16A (SL18539R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD

Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (SL18539R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(SL18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(SL18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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