Protein: k562(human) lysate at 40ug;
Primary: rabbit Anti-phospho-MAK (Tyr159) (SL18634R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size: 71 kD
Observed band size: 71 kD
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- phospho-MAK (Tyr159) (SL18634R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 73 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- phospho-MAK (Tyr159) (SL18634R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71/66 kD
Tissue/cell: human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated(SL18634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated(SL18634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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