Rabbit Anti-DHRS2 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-DHRS2 antibody

Dehydrogenase/reductase (SDR family) member 2; Dehydrogenase/reductase member 2; Dehydrogenase/reductase SDR family member 2; Dicarbonyl reductase HEP27; DHRSⅡ; Hep27; HEP27 protein; Protein D; SDR family member; SDR25C1; Short chain alcohol dehydrogenase

Cat:

SL10771R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human DHRS2:61-160/258

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Protein: Hela(human) lysates at 40ug; Primary: rabbit Anti-DARC (SL10771R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size:27 kDObserved band size: 30 kDProtein: lung(mouse) lysates at 40ug;Primary: Anti-DHRS2 (SL10771R) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size :27 kDObserved band size :27 kDProtein: liver(mouse) lysates at 30 ug; Primary: Anti-DHRS2 (SL10771R) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 27 kDObserved band size : 27/75 kDTissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(SL10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(SL10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Picture

Protein: lung(mouse) lysates at 40ug; Primary: Anti-DHRS2 (SL10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :27 kD
Observed band size :27 kD

Protein: liver(mouse) lysates at 30 ug;
Primary: Anti-DHRS2 (SL10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 27 kD
Observed band size : 27/75 kD

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(SL10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(SL10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall