Home > Product > Antibody > Rabbit Anti-OMA1 antibody
2010001O09Rik; DAB1; FLJ33782; Metalloendopeptidase OMA1; Metalloendopeptidase OMA1, mitochondrial; Metalloprotease-related protein 1; mitochondrial; MPRP 1; MPRP-1; MPRP1; OMA1; OMA1 homolog zinc metallopeptidase; OMA1 homolog, zinc metallope
Cat:
SL19641R
Species Reactivity:
Human,Mouse,Rat,(predicted: Chicken,Pig,Cow,Horse,Rabbit,Zebrafish,Cat,)
Immunogen:
KLH conjugated synthetic peptide derived from human OMA1:401-500/524
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Heart (Rat) Lysate at 40 ugPrimary: Anti- OMA1 (SL19641R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60 kDObserved band size: 55 kDSample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti- OMA1 (SL19641R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60 kDObserved band size: 55 kDSample: TT Cell (Human) Lysate at 40 ugPrimary: Anti- OMA1 (SL19641R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60 kDObserved band size: 55 kDSample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- OMA1 (SL19641R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60 kDObserved band size: 55 kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OMA1) Polyclonal Antibody, Unconjugated (SL19641R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample: Heart (Rat) Lysate at 40 ug
Primary: Anti- OMA1 (SL19641R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD
Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti- OMA1 (SL19641R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD
Sample:
TT Cell (Human) Lysate at 40 ug
Primary: Anti- OMA1 (SL19641R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- OMA1 (SL19641R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OMA1) Polyclonal Antibody, Unconjugated (SL19641R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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