Rabbit Anti-PPP3CC antibody

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calcineurin A gamma; CALCINEURIN A3; Calcineurin; Calcineurin testis specific catalytic subunit; Calmodulin dependent calcineurin A subunit gamma isoform; Calmodulin-dependent calcineurin A subunit gamma isoform; CALNA3; CAM PRP catalytic subunit;

Cat:

SL19973R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human PPP3CC:51-150/512

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis(Mouse)Lysate at 40 ugPrimary: Anti-PPP3CC(SL19973R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 58kDObserved band size: 58kDSample: A549 (Human)Cell Lysate at 40 ugPrimary: Anti-PPP3CC(SL19973R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 58kDObserved band size: 58kDSample: Hela(Human) Cell Lysate at 40 ugPrimary: Anti-PPP3CC (SL19973R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 58 kDObserved band size: 58 kDSample: DU145(Human) Cell Lysate at 40 ugPrimary: Anti-PPP3CC (SL19973R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 58 kDObserved band size: 58 kDParaformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated (SL19973R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Calcineurin is a calcium-dependent, calmodulin-stimulated protein phosphatase involved in the downstream regulation of dopaminergic signal transduction. Calcineurin is composed of a regulatory subunit and a catalytic subunit. The protein encoded by this gene represents one of the regulatory subunits that has been found for calcineurin. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Function:
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Subunit:
Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Tissue Specificity:
Testis.

Similarity:
Belongs to the PPP phosphatase family. PP-2B subfamily.

SWISS:
P48454

Gene ID:
5533

Database links:

Entrez Gene: 5533 Human

Entrez Gene: 19057 Mouse

Entrez Gene: 171378 Rat

Omim: 114107 Human

SwissProt: P48454 Human

SwissProt: P48455 Mouse

Unigene: 728861 Human

Unigene: 731683 Human

Unigene: 439683 Mouse

Picture

Sample: Testis(Mouse)Lysate at 40 ug
Primary: Anti-PPP3CC(SL19973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD

Sample: A549 (Human)Cell Lysate at 40 ug
Primary: Anti-PPP3CC(SL19973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD

Sample:
Hela(Human) Cell Lysate at 40 ug
Primary: Anti-PPP3CC (SL19973R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD

Sample:
DU145(Human) Cell Lysate at 40 ug
Primary: Anti-PPP3CC (SL19973R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated (SL19973R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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