Rabbit Anti-CK5+CK6 antibody

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CK5+6; Cytokeratin 5 + 6; Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; CK 5; DDD; EBS 2; EBS2; K5; Keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin Ty

Cat:

SL20824R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthetic peptide derived from human CK5+CK6:161-220/590

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Esophagus (Mouse) Lysate at 40 ugPrimary: Anti-CK5+CK6 (SL20824R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62/60 kDObserved band size: 62/60 kDSample: HepG2(Human) Cell Lysate at 30 ugPrimary: Anti-CK5+CK6 (SL20824R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62/60 kDObserved band size: 62/60 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (SL20824R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (SL20824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃). Primary Antibody (green line): Rabbit Anti-CK5+CK6 antibody (SL20824R), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Keratins 5, 6 are members of type II keratins family that are specifically expressed in the inner root sheath of hair follicles. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin 18 is memeber of the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family.

SWISS:
P02538

Gene ID:
3852

Database links:

Entrez Gene: 3852 Human

Entrez Gene: 3853 Human

Entrez Gene: 3854 Human

Omim: 29640 Human

SwissProt: P02538 Human

SwissProt: P04259 Human

SwissProt: P13647 Human

细胞角蛋白5&6是高分子量细胞角蛋白，分子量分别为58KDa和56KDa。其表达在皮肤的基底细胞、棘层细胞和部分前列腺基底细胞。主要用于间皮瘤和腺癌的鉴别诊断，并可辅助诊断前列腺良恶性病变。 Picture

Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-CK5+CK6 (SL20824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62/60 kD
Observed band size: 62/60 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (SL20824R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (SL20824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃).
Primary Antibody (green line): Rabbit Anti-CK5+CK6 antibody (SL20824R), Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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