Rabbit Anti-SOD2 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-SOD2 antibody

IPO B; Manganese SOD; Superoxide Dismutase 2; Manganese superoxide dismutase; Mn SOD; MNSOD; SOD 2; SOD-2; SOD2; Superoxide dismutase [Mn] mitochondrial; Superoxide dismutase [Mn] mitochondrial precursor; Superoxide dismutase 2 mitochondrial; SODM_HUMAN.

Cat:

SL23402R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human SOD2:21-120/222

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Kidney (Mouse) Lysate at 40 ugLane 3: Testis (Mouse) Lysate at 40 ugLane 4: Liver (Mouse) Lysate at 40 ugLane 5: Kidney (Rat) Lysate at 40 ugLane 6: Testis (Rat) Lysate at 40 ugLane 7: Pancreas (Rat) Lysate at 40 ugLane 8: Liver (Rat) Lysate at 40 ugPrimary: Anti-SOD2 (SL23402R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kDample: Kidney (Mouse) Lysate at 40 ugStomach (Mouse) Lysate at 40 ugPrimary: Anti- SOD2 (SL23402R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kDSample: Kidney(Mouse) Lysate at 40 ugPrimary: Anti-SOD2 (SL23402R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 27 kDSample: Cerebrum (Rat) Lysate at 40 ugHeart (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugPrimary: Anti-SOD2 (SL23402R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kDParaformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Function:
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Subunit:
Homotetramer.

Subcellular Location:
Mitochondrion matrix.

Post-translational modifications:
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting.

DISEASE:
Microvascular complications of diabetes 6 (MVCD6) [MIM:612634]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Similarity:
Belongs to the iron/manganese superoxide dismutase family.

SWISS:
P04179

Gene ID:
6648

Database links:

Entrez Gene: 374042 Chicken

Entrez Gene: 281496 Cow

Entrez Gene: 476258 Dog

Entrez Gene: 6648 Human

Entrez Gene: 20656 Mouse

Entrez Gene: 100154319 Pig

Entrez Gene: 24787 Rat

Omim: 14792 Human

SwissProt: P41976 Cow

SwissProt: P04179 Human

SwissProt: P09671 Mouse

SwissProt: P41982 Rabbit

SwissProt: P07895 Rat

Unigene: 487046 Human

Unigene: 290876 Mouse

Unigene: 10488 Rat

Picture

ample:
Kidney (Mouse) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- SOD2 (SL23402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD

Sample:
Kidney(Mouse) Lysate at 40 ug
Primary: Anti-SOD2 (SL23402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 27 kD

Sample:
Cerebrum (Rat) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-SOD2 (SL23402R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOD2) Polyclonal Antibody, Unconjugated (SL23402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall