Rabbit Anti-5HT1A Receptor antibody

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5HT1A Receptor; HTR1A; 5-HTR1A; 5 HT 1A; 5 HT receptor 1A; 5 HT1A; 5 HT1A receptor; 5 hydroxytryptamine (serotonin) receptor 1A; 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled; 5 hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; 5-hydroxytryp

Cat:

SL1124R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human 5-HTR1A:301-400/422<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Protein: Brain(Mouse) Lysate at 40 ugPrimary: Rabbit Anti- 5HT1A Receptor (SL1124R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:46 kDObserved band size:56 kDProtein: HepG2 lyates(hu); Primary: Rabbit Anti- 5HT1A Receptor (SL1124R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:46 kDObserved band size:56 kDSample: Lane 1: Mouse Brain Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Mouse Lymph Lysates Lane 4: Rat Brain Lysates Lane 5: Rat Cerebellum Lysates Primary: Anti-5HT1A Receptor (SL1124R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 46kDaObserved band size: 46kDaSample: Jurkat(Human) Cell Lysate at 40 ugPrimary: Anti-5HT1A Receptor (SL1124R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 46 kDObserved band size: 46 kDTissue/cell: rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-5HT1A Receptor Polyclonal Antibody, Unconjugated(SL1124R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012].

Function:
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

SWISS:
P19327

Gene ID:
3350

Database links:

Entrez Gene: 487230 Dog

Entrez Gene: 3350 Human

Entrez Gene: 15550 Mouse

Entrez Gene: 24473 Rat

Omim: 109760 Human

SwissProt: Q6XXX9 Dog

SwissProt: Q0EAB6 Horse

SwissProt: P08908 Human

SwissProt: Q64264 Mouse

SwissProt: P19327 Rat

Unigene: 247940 Human

Unigene: 4716 Mouse

Unigene: 44486 Rat

Picture

Protein: HepG2 lyates(hu);
Primary: Rabbit Anti- 5HT1A Receptor (SL1124R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:46 kD Observed band size:56 kD

Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Mouse Lymph Lysates
Lane 4: Rat Brain Lysates
Lane 5: Rat Cerebellum Lysates
Primary: Anti-5HT1A Receptor (SL1124R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46kDa
Observed band size: 46kDa

Sample:
Jurkat(Human) Cell Lysate at 40 ug
Primary: Anti-5HT1A Receptor (SL1124R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD

Tissue/cell: rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-5HT1A Receptor Polyclonal Antibody, Unconjugated(SL1124R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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