Rabbit Anti-ADRB1 antibody | Sunlong Medical

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beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; Beta 1 adrenoceptor; Beta 1 adrenoreceptor; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB

Cat:

SL0498R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human ADRB1:181-250/477<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 30 ugHeart (Rat) Lysate at 30 ugPrimary: Anti- ADRB1(SL0498R) at 1/200 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/3000 dilutionPredicted band size: 52 kDObserved band size: 55 kDTissue/cell: skin of rat foot; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(SL0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(SL0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control:U937. Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (SL0498R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control:U937. Primary Antibody (green line): Rabbit Anti- antibody (SL3152R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Tissue/cell: mouse kidney cell; Incubation: Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated(SL0498R-FITC) 1:200, 40 minutes at 37°C. Green line: Isotype Control(SL0295P-FITC, rabbit IgG/FITC) 1:200, 40 minutes at 37°C.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family.

Function:
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.

Subunit:
Interacts with GOPC, MAGI3 and DLG4.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.

Post-translational modifications:
Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

Similarity:
Belongs to the G-protein coupled receptor 1 family.
Adrenergic receptor subfamily. ADRB1 sub-subfamily.

SWISS:
P08588

Gene ID:
153

Database links:

Entrez Gene: 153 Human

Entrez Gene: 11554 Mouse

Entrez Gene: 24925 Rat

Omim: 109630 Human

SwissProt: P08588 Human

SwissProt: P34971 Mouse

SwissProt: P3690 Rat

Unigene: 99913 Human

Unigene: 46797 Mouse

Unigene: 87064 Rat

β1肾上腺素能受体（Beta-adrenergic Receptor-1 ）在接受交感神经节后纤维支配的各种器官中存在着与肾上腺素、去甲肾上腺素起反应的受体，称为肾上腺素能受体。其化学性质尚不清楚。用对药物反应的方法，肾上腺素能受体可分为α及β两个类型。肾上腺素对α、β及D型受体均起作用，而去甲肾上腺素主要对α型起作用。
β1AR所引起的反应为支气管扩张、血管扩张等，肾上腺素受体可被β13，4－dichloroisoproterenol（D－CI）等阻断药物所抑制。 Picture

Tissue/cell: skin of rat foot; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(SL0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(SL0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control:U937.
Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (SL0498R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:U937.
Primary Antibody (green line): Rabbit Anti- antibody (SL3152R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: mouse kidney cell;
Incubation:
Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated(SL0498R-FITC) 1:200, 40 minutes at 37°C.
Green line: Isotype Control(SL0295P-FITC, rabbit IgG/FITC) 1:200, 40 minutes at 37°C.

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Specific References (7) | SL0498R has been referenced in 7 publications.

[IF=4.831] Liu Z et al. Over-expression of microRNA-145 drives alterations in β-adrenergic signaling and attenuates cardiac remodeling in heart failure post myocardial infarction. Aging (Albany NY). 2020 Jun 18;12(12):11603-11622. WB ; Rat.

PubMed:32554856

[IF=2.71] Bornholz, Beatrice, et al. "A standardised FACS assay based on native, receptor transfected cells for the clinical diagnosis and monitoring of β1-adrenergic receptor autoantibodies in human heart disease." Clinical Chemistry and Laboratory Medicine (CCLM). Human.

PubMed:21288610

[IF=3.07] Deng, Guo-Hua, et al. "Exogenous norepinephrine attenuates the efficacy of sunitinib in a mouse cancer model." Journal of Experimental & Clinical Cancer Research 33:21 (2014). IHSLCP ; Mouse.

PubMed:24555849

[IF=3.727] Yue Wang. et al. Mirabegron Ameliorated Atherosclerosis of ApoE−/− Mice in Chronic Intermittent Hypoxia but Not in Normoxia. 2021 Jun 21 IHC ; Mouse.

PubMed:34152510

[IF=2.5] Jinyao Liuet al. Alcohol consumption combined with dietary low-carbohydrate/high-protein intake increased the left ventricular systolic dysfunction risk and lethal ventricular arrhythmia susceptibility in apolipoprotein E/low-density lipoprotein receptor double-knockout mice. Alcohol . 2020 Dec;89:63-74. IHC ; mouse.

PubMed:32702503

[IF=2.078] Shi L et al. β1 adrenoceptor antibodies induce myocardial apoptosis via inhibiting PGSLC1α-related pathway. BMC Cardiovasc Disord. 2020 Jun 5;20(1):269. WB ; Rat.

PubMed:32503464

[IF=3.14] Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032. WB ; rat.

PubMed:32213114