Rabbit Anti-Angiotensin II Type 1 Receptor antibody

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AG2S; Agtr 1; Agtr1; Agtr1a; AGTR1B; Angiotensin II receptor type 1; Angiotensin II type 1 receptor; AT-1B; AT-1r; AT1; At1a; AT1AR; AT1B; AT1BR; AT2R1; AT2R1A; AT2R1B; HAT1R; Type 1 angiotensin II receptor; AGTR1_HUMAN; Type-1 angiotensin II receptor; An

Cat:

SL0630R

Species Reactivity：

Human,Mouse,Rat,(predicted: Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human AT1R:151-250/359<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (mouse) Lysate at 40 ugPrimary: Anti- Angiotensin II Type 1 Receptor (SL0630R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39kDObserved band size: 36kDSample: HepG2 (human)cell Lysate at 40 ugPrimary: Anti- Angiotensin II Type 1 Receptor (SL0630R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39kDObserved band size: 49kDTissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated(SL0630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat myocardium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated(SL0630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least five transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants. [provided by RefSeq].

Function:
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit:
Interacts with MAS1 (Probable). Interacts with ARRB1.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Liver, lung, adrenal and adrenocortical adenomas.

Post-translational modifications:
SLCterminal Ser or Thr residues may be phosphorylated.

DISEASE:
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P30556

Gene ID:
185

Database links:

Entrez Gene: 185 Human

Entrez Gene: 11607 Mouse

Entrez Gene: 11608 Mouse

Entrez Gene: 2436 Rat

Entrez Gene: 81638 Rat

Omim: 106165 Human

SwissProt: P30556 Human

SwissProt: P29754 Mouse

SwissProt: P29755 Mouse

SwissProt: P25095 Rat

SwissProt: P29089 Rat

Unigene: 477887 Human

Unigene: 728754 Human

AT1-受体：血管紧张素Ⅱ-1型受体抗体,主要用于心血管病方面的研究。 Picture

Sample: HepG2 (human)cell Lysate at 40 ug
Primary: Anti- Angiotensin II Type 1 Receptor (SL0630R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 49kD

Tissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated(SL0630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat myocardium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated(SL0630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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