Rabbit Anti-PD-L1 antibody | Sunlong Medical

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Programmed cell death 1 ligand 1; CD274; B7 H; B7 H1; B7 homolog 1; B7-H1; B7H; B7H1; CD 274; CD274 antigen; CD274 molecule; MGC142294; MGC142296; PD L1; PD1L1_HUMAN; PD1L1_Mouse; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL 1; PDL1; Progra

Cat:

SL1103R

Species Reactivity：

Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse B7-H1:201-290/290

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti- CD274 (SL1103R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilutionPredicted band size: 32 kDObserved band size: 48 kDSample: Lymph node (Mouse) Lysate at 40 ugPrimary: Anti- CD274 (SL1103R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilutionPredicted band size: 32 kDObserved band size: 48 kDTissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-CD274 Polyclonal Antibody, Unconjugated(SL1103R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: Hela. Primary Antibody (green line): Rabbit Anti-PD-L1 antibody (SL1103R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin SLVlike and SLClike domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Function:
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Subunit:
Interacts with PDCD1.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Endomembrane system; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

Similarity:
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q9EP73

Gene ID:
60533

Database links:

Entrez Gene: 29126 Human

Entrez Gene: 60533 Mouse

Entrez Gene: 499342 Rat

Omim: 605402 Human

SwissProt: Q9NZQ7 Human

SwissProt: Q9EP73 Mouse

Unigene: 521989 Human

Unigene: 245363 Mouse

Unigene: 228198 Rat

程序性死亡配体1（B7-H1）可以促进上皮细胞的恶性转化,保护表皮细胞免于失巢凋亡,在肿瘤的发生,进展和转归中发挥重要作用.
目前对CD274信号通路的进一步的研究必将为自身免疫性疾病、病毒感染性疾病和器官移植后排斥反应中T细胞介导的免疫应答调控提供了新的途径.B7-H1在癌症、类风湿、HIV感染等疾病中发现B7-H1有负调节作用。对B7-H1路径进行调控有助于自身免疫病及恶性肿瘤的治疗。 Picture

Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- CD274 (SL1103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 32 kD
Observed band size: 48 kD

Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-CD274 Polyclonal Antibody, Unconjugated(SL1103R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-PD-L1 antibody (SL1103R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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