[email protected]
Home > Product > Antibody > Rabbit Anti-BDNF antibody
Abrineurin; Brain Derived Neurotrophic Factor; MGC34632; Neurotrophin; BDNF_HUMAN.
Cat:
SL0248R
Species Reactivity：
Human,Rat,Rabbit,(predicted: Mouse,Dog,Pig,Cow,Horse,Guinea Pig,)
Immunogen：
KLH conjugated synthetic peptide derived from human BDNF:81-180/247
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample:Brain(Rat)lysate at 30ug;Heart(Rat)lysates, 30ugPrimary: Anti-BDNF (SL0248R) at 1:200Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000Predicted band size : 13kD,25kDObserved band size : 25kDTissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated(SL0248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated(SL0248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: HepG2(blue). Primary Antibody:Rabbit Anti-BDNF antibody (SL0248R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0248R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.
More
prev next
Unit:
Price: $
Product PDFs
Datasheet:


Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. More recently, three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4), also designated NT-5. These various neurotrophins stimulate the in vitro survival of distinct but partially overlapping populations of neurons. The Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds equally well to both BDNF and NT-4 and to a lesser extent NT-3, while the Trk C receptor only binds NT-3. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Belongs to the NGF-beta family.

Function:
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Subunit:
Monomers and homodimers. Binds to NTRK2/TRKB.

Subcellular Location:
Secreted.

Tissue Specificity:
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Post-translational modifications:
The propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).

DISEASE:
Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209176]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.

Similarity:
Belongs to the NGF-beta family.

SWISS:
P23560

Gene ID:
627

Database links:

Entrez Gene: 627 Human

Entrez Gene: 12064 Mouse

Entrez Gene: 24225 Rat

Omim: 113505 Human

SwissProt: P23560 Human

SwissProt: P21237 Mouse

SwissProt: P23363 Rat

Unigene: 502182 Human

Unigene: 1442 Mouse

Unigene: 11266 Rat



神经生物学相关蛋白（Neurobiology）
人脑源性神经营养因子(BDNF)和神经营养素-3(NT-3)神经生长因子(NGF)属于神经营养因子(NTF)家族的组成成员，具有促进早期神经元的增殖与分化，维持成熟神经元的存活的生物学功能,能调控神经元再生的微环境、可促进运动神经元的存活和生长并能防止它受损及死亡。对治疗神经系统疾病有广泛的临床应用前景。
目前已经用于某些疾病的临床试验：如早期老年性痴呆症、帕金森氏症、糖尿病、艾滋病或其他原因引起的外周神经损伤等研究。 Picture

Sample:
Brain(Rat)lysate at 30ug;
Heart(Rat)lysates, 30ug
Primary: Anti-BDNF (SL0248R) at 1:200
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000
Predicted band size : 13kD,25kD
Observed band size : 25kD
Tissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated(SL0248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated(SL0248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Blank control: HepG2(blue).
Primary Antibody:Rabbit Anti-BDNF antibody (SL0248R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0248R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.