Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
Function:
Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.
Subunit:
Monomer.
Subcellular Location:
Secreted, extracellular space.
Tissue Specificity:
Present in articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Similarity:
Belongs to the glycosyl hydrolase 18 family.
SWISS:
Q9WTV1
Gene ID:
89824
Database links:
Entrez Gene: 1116 Human
Entrez Gene: 12654 Mouse
Entrez Gene: 396865 Pig
Entrez Gene: 89824 Rat
Omim: 601525 Human
SwissProt: P36222 Human
SwissProt: Q61362 Mouse
SwissProt: Q5RBP6 Orangutan
SwissProt: Q29411 Pig
SwissProt: Q9WTV1 Rat
Unigene: 382202 Human
Unigene: 38274 Mouse
Unigene: 68940 Rat
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Protein:
Liver(Rat) lysate at 30ug;
Colon carcinoma(Human) lysates at 30ug;
Primary: Anti-CGP-39/CHI3L1/YKL-40 (SL1093R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000;
Predicted band size : 42kD
Observed band size : 39kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated(SL1093R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated(SL1093R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CGP-39/YKL-40 Polyclonal Antibody, Unconjugated(SL1093R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (SL1093R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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