Rabbit Anti-Calponin 1 antibody

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Basic calponin; Calponin-1; Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin1; Calponins basic; CNN 1; CNN1; Sm Calp; SMCC; CNN1_HUMAN; Calponin H1; Calponin1; Sm Calp; smooth muscle.

Cat:

SL0095R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Calponin 1:201-297/297

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Urinary bladder (Mouse) Lysate at 40 ugPrimary: Anti-Calponin 1 (SL0095R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 33 kDObserved band size: 33 kDTissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(SL0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(SL0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingPositive control: H9C2(2% Paraformaldehyde-fixed )Isotype Control AntibodyAntibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSASecondary AntibodyAntibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary AntibodySupplier catalog number: SL0095R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Predicted to enable actin binding activity. Involved in negative regulation of vascular associated smooth muscle cell proliferation. Located in cytoskeleton. [provided by Alliance of Genome Resources, Nov 2021]

Function:
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Subunit:
Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.

Tissue Specificity:
Smooth muscle, and tissues containing significant amounts of smooth muscle.

Similarity:
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.

SWISS:
P51911

Gene ID:
1264

Database links:

Entrez Gene: 1264 Human

Entrez Gene: 484937 Dog

Entrez Gene: 12797 Mouse

Entrez Gene: 396911 Pig

Entrez Gene: 65204 Rat

Omim: 600806 Human

SwissProt: P51911 Human

SwissProt: Q08091 Mouse

SwissProt: Q08092 Pig

SwissProt: Q08290 Rat

Unigene: 465929 Human

Unigene: 4356 Mouse

Unigene: 31788 Rat

Calponin1是一种激动蛋白结合蛋白，是平滑肌特有的一种蛋白，可以与肌动蛋白、原肌球蛋白和钙调素结合,具有抑制平滑肌细胞收缩的功能，主要用于平滑肌肿瘤和乳腺等组织中的肌上皮细胞分布的研究。 Picture

Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(SL0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(SL0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Positive control: H9C2(2% Paraformaldehyde-fixed )
Isotype Control Antibody
Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier catalog number: SL0095R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

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