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Home > Product > Antibody > Rabbit Anti-Cyclin D2 antibody
CCND 2; CCND2; CyclinD2; G1/S specific cyclin D2; KIAK0002; MGC102758; CCND2_HUMAN; G1/S-specific cyclin-D2.
Cat:
SL1148R
Species Reactivity：
Human,Rat,(predicted: Mouse,)
Immunogen：
KLH conjugated synthetic peptide derived from the middle of human Cyclin D2:161-288/288
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=5μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated(SL1148R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human flat moss tinea tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated(SL1148R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat mammary tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated(SL1148R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiThe figure annotation: The blue histogram is unstained cells.The Wathet Blue histogram is cells stained with secondary antibody(SL0295G-FITC) alone.The Orange histogram is cells stained with rabbit IgG isotype control(SL0295P) antibody plus secondary antibody.The green histogram is cells stained with Rabbit Anti-Cyclin D2 antibody (SL1148R)plus secondary antibody..Concebtration: 5μg/10^6 cells.Positive control: MCF-7 cells.
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Product PDFs
Datasheet:


The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

Function:
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Subunit:
Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity.

Subcellular Location:
Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.

Similarity:
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.

SWISS:
P30279

Gene ID:
894

Database links:

Entrez Gene: 894 Human

Entrez Gene: 12444 Mouse

Entrez Gene: 12833 Rat

Omim: 123833 Human

SwissProt: P30279 Human

SwissProt: P30280 Mouse

SwissProt: Q04827 Rat

Unigene: 376071 Human

Unigene: 333406 Mouse

Unigene: 96083 Rat



Cyclin D2与周期素D1一样，为细胞周期中G1期进入S期的一个重要调控因子。细胞周期素是周期素依赖激酶的调节因子，不同的细胞周期素控制着细胞周期中不同的特定阶段。Cyclin D2通过激活CdK4或CdK6等，促进DNA合成，加速细胞增殖。主要用于B细胞淋巴瘤、乳腺癌、头颈部鳞癌、食道癌、肝癌和肺癌等恶性肿瘤的研究。 Picture

Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated(SL1148R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human flat moss tinea tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated(SL1148R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat mammary tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated(SL1148R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei
The figure annotation: The blue histogram is unstained cells. The Wathet Blue histogram is cells stained with secondary antibody(SL0295G-FITC) alone. The Orange histogram is cells stained with rabbit IgG isotype control(SL0295P) antibody plus secondary antibody. The green histogram is cells stained with Rabbit Anti-Cyclin D2 antibody (SL1148R)plus secondary antibody. .
Concebtration: 5μg/10^6 cells.
Positive control: MCF-7 cells.
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