Rabbit Anti-IL-9 antibody | Sunlong Medical

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Interleukin-9; IL9; Homolog of mouse T cell and mast cell growth factor 40; HP40; IL 9; Mast cell growth factor; MCGF; Megakaryoblast growth factor; p40 cytokine; p40 T cell and mast cell growth factor; p40 T-cell and mast cell growth factor; T cell grow

Cat:

SL2428R

Species Reactivity：

Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse IL-9:21-120/144

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:200-800（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-IL-9 (SL2428R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 16 kDObserved band size: 16 kDParaformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (SL2428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated(SL2428R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingParaformaldehyde-fixed, paraffin embedded (Mouse mammary tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (SL2428R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a cytokine that actsas a regulator of a variety of hematopoietic cells. This cytokinestimulates cell proliferation and prevents apoptosis. It functionsthrough the interleukin 9 receptor (IL9R), which activatesdifferent signal transducer and activator (STAT) proteins and thusconnects this cytokine to various biological processes. The geneencoding this cytokine has been identified as a candidate gene forasthma. Genetic studies on a mouse model of asthma demonstratedthat this cytokine is a determining factor in the pathogenesis ofbronchial hyperresponsiveness. [provided by RefSeq, Jul 2008].

Function:
Supports IL-2 independent and IL-4 independent growth of helper T-cells.

Subcellular Location:
Secreted.

Similarity:
Belongs to the IL-7/IL-9 family.

SWISS:
P15247

Gene ID:
16198

Database links:

Entrez Gene: 3578 Human

Entrez Gene: 16198 Mouse

Omim: 146931 Human

SwissProt: P15248 Human

SwissProt: P15247 Mouse

Unigene: 960 Human

Unigene: 3006 Mouse

白介素-9是由CD4和辅助性T细胞的Th2细胞分泌的细胞因子,具有多种生物学活性，在机体的免疫应答中具有多种免疫调节作用，IL-9参与肿瘤发生、寄生虫免疫、支气管哮喘、慢性阻塞性肺病和器官移植等多种疾病过程。 Picture

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-IL-9 (SL2428R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16 kD
Observed band size: 16 kD

Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (SL2428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated(SL2428R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Paraformaldehyde-fixed, paraffin embedded (Mouse mammary tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (SL2428R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.

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