Rabbit Anti-HRAS antibody | Sunlong Medical

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c bas/has; c H ras; c has/bas p21 protein; C K RAS; c K ras2 protein; c Ki ras; c Kirsten ras protein; c ras Ki 2 protein; Cellular c Ki ras2 proto oncogene antibody G1III6 N ras; GTPase HRas; GTPase KRas; GTPase NRas; H Ras 1; H RasIDX; Ha Ras; HRAS 1; H

Cat:

SL1071R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human H-ras:101-152/189

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-HRAS/Ras/Ras p21 Polyclonal Antibody, Unconjugated(SL1071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-HRAS/Ras/Ras p21 Polyclonal Antibody, Unconjugated(SL1071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq].

Function:
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Subunit:
In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3. Interacts with HSPD1. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain) (By similarity). Forms a signaling complex with RASGRP1 and DGKZ. Interacts with RASSF5. Interacts with PDE6D. Interacts with IKZF3. Interacts with GNB2L1.

Subcellular Location:
Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
Isoform 2: Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Colocalizes with GNB2L1 to the perinuclear region.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuouscycle of de- and re-palmitoylation regulates rapid exchange betweenplasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important forstimulating guanine nucleotide exchange. No structural perturbationon nitrosylation.
The covalent modification of cysteine by15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic andreversible. It may occur as an alternative to other cysteinemodifications, such as S-nitrosylation and S-palmitoylation.
Acetylation at Lys-104 prevents interaction with guaninenucleotide exchange factors (GEFs) (By similarity).

DISEASE:
Faciocutaneoskeletal syndrome (FCSS) [MIM:23640]: A rarecondition characterized by prenatally increased growth, postnatalgrowth deficiency, mental retardation, distinctive facialappearance, cardiovascular abnormalities (typically pulmonicstenosis, hypertrophic cardiomyopathy and/or atrial tachycardia),tumor predisposition, skin and musculoskeletal abnormalities.Note=The disease is caused by mutations affecting the generepresented in this entry.
Congenital myopathy with excess of muscle spindles(CMEMS) [MIM:23640]: Variant of Costello syndrome. Note=Thedisease is caused by mutations affecting the gene represented inthis entry.
Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]: Arare type of thyroid cancer accounting for only about 3-10% of alldifferentiated thyroid cancers. These neoplasms are considered avariant of follicular carcinoma of the thyroid and are referred toas follicular carcinoma, oxyphilic type. Note=Diseasesusceptibility is associated with variations affecting the generepresented in this entry.
Note=Mutations which change positions 12, 13 or 61activate the potential of HRAS to transform cultured cells and areimplicated in a variety of human tumors.
Bladder cancer (BLC) [MIM:22000]: A malignancyoriginating in tissues of the urinary bladder. It often presentswith multiple tumors appearing at different times and at differentsites in the bladder. Most bladder cancers are transitional cellcarcinomas that begin in cells that normally make up the innerlining of the bladder. Other types of bladder cancer includesquamous cell carcinoma (cancer that begins in thin, flat cells)and adenocarcinoma (cancer that begins in cells that make andrelease mucus and other fluids). Bladder cancer is a complexdisorder with both genetic and environmental influences.Note=Disease susceptibility is associated with variations affectingthe gene represented in this entry.
Note=Defects in HRAS are the cause of oral squamous cellcarcinoma (OSCC).
Schimmelpenning-Feuerstein-Mims syndrome (SFM)[MIM:16640]: A disease characterized by sebaceous nevi, often onthe face, associated with variable ipsilateral abnormalities of thecentral nervous system, ocular anomalies, and skeletal defects.Many oral manifestations have been reported, not only includinghypoplastic and malformed teeth, and mucosal papillomatosis, butalso ankyloglossia, hemihyperplastic tongue, intraoral nevus, giantcell granuloma, ameloblastoma, bone cysts, follicular cysts,oligodontia, and odontodysplasia. Sebaceous nevi follow the linesof Blaschko and these can continue as linear intraoral lesions, asin mucosal papillomatosis. Note=The disease is caused by mutationsaffecting the gene represented in this entry.

Similarity:
Belongs to the small GTPase superfamily. Ras family.

SWISS:
P01112

Gene ID:
3265

Database links:

Entrez Gene: 3265 Human

Entrez Gene: 15461 Mouse

Entrez Gene: 293621 Rat

Omim: 190020 Human

SwissProt: P01112 Human

SwissProt: Q61411 Mouse

SwissProt: P20171 Rat

Unigene: 37003 Human

Unigene: 334313 Mouse

Unigene: 10236 Rat

GDP/GTP结合蛋白（GDP/GTP Bijding Protein）
细胞信号转导系统紊乱是肿瘤细胞生长的重要特征之一，Ras蛋白参与体内多种细胞信号转导途径而发挥作用,而原癌基因H-ras是一种多功能的细胞因子，广泛存在于自然界，H-ras在多种细胞生命活动中起极为重要的作用，包括细胞的增殖、分化和细胞骨架的构建等等，该抗体主要用于肿瘤方面的研究。 Picture

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-HRAS/Ras/Ras p21 Polyclonal Antibody, Unconjugated(SL1071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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