Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Summary: catalyzes the conversion of heme to biliverdin; involved in cellular response to oxidative stress [SUBCELLULAR LOCATION] Microsome. Endoplasmic reticulum. [INDUCTION] Heme oxygenase 2 activity is non-inducible. [SIMILARITY] Belongs to the heme oxygenase family.
Function:
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Subcellular Location:
Microsome. Endoplasmic reticulum.
Similarity:
Belongs to the heme oxygenase family.
Contains 2 HRM (heme regulatory motif) repeats.
SWISS:
P30519
Gene ID:
3163
Database links:
Entrez Gene: 510243 Cow
Entrez Gene: 479864 Dog
Entrez Gene: 3163 Human
Entrez Gene: 15369 Mouse
Entrez Gene: 100009523 Rabbit
Entrez Gene: 79239 Rat
Omim: 141251 Human
SwissProt: P30519 Human
SwissProt: O70252 Mouse
SwissProt: P43242 Rabbit
SwissProt: P23711 Rat
Unigene: 284279 Human
Unigene: 272866 Mouse
Unigene: 10241 Rat
合成与降解（Synthesis and Degradation）
血红素氧合酶是目前发现生物体内最易被诱导产生的抗氧化酶类，其抗氧化作用与它的催化分解产物即胆绿素、胆红素、NO、铁离子和铁蛋白有关。
血红素氧合酶-2基因缺失对血红素诱导的氧化应激性导致的组织损伤具有保护作用;选择性抑制神经元血红素氧合酶-2基因的表达可减轻氧化应激性组织细胞损伤.
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Sample:
Brain(Rat)lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-HO-2 (SL1238R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 38kD
Observed band size : 38kD, 48kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- HO-2 (SL1238R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HO-2) Polyclonal Antibody, Unconjugated (SL1238R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HO-2 Antibody(SL1238R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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