Rabbit Anti-DFFA antibody | Sunlong Medical

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DNA fragmentation factor subunit alpha; DFF-45; Inhibitor of CAD; ICAD; DFF1; DFF45; H13; DFFA; DFFA_HUMAN;

Cat:

SL0214R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human ICAD:2-100/331

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: liver (mouse) Lysate at 40 uglung(mouse) Lysate at 40 ugBRL-3A (rat)cell Lysate at 40 ugPrimary: Anti- ICAD (SL0214R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36kDObserved band size: 36 kDSample: A431 Cell (Human) Lysate at 40 ugPrimary: Anti-ICAD (SL0214R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDTissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ICAD Polyclonal Antibody, Unconjugated(SL0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ICAD/DFF-45 Beta Polyclonal Antibody, Unconjugated(SL0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Caspase-activated deoxyribonuclease(CAD) also designated DNA fragmentation factor 45 kDa subunit (DFF-45)and Caspase-activated DNase. CAD is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. ICAD (DNA fragmentation factor alpha subunit) (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) is inhibitor of the caspase-activated DNase (DFF44).

Function:
Inhibitor of the caspase-activated DNase (DFF40).

Subunit:
Heterodimer of DFFA and DFFB.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.

Similarity:
Contains 1 CIDE-N domain.

SWISS:
O54786

Gene ID:
1676

Database links:

Entrez Gene: 1676 Human

Omim: 601882 Human

SwissProt: O00273 Human

Unigene: 484782 Human

ICAD 是在细胞凋亡期诱导DNA分裂和染色体固缩的一种核酶，降解裸露的DNA 并诱导形态学变化。 Picture

Sample: A431 Cell (Human) Lysate at 40 ug
Primary: Anti-ICAD (SL0214R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ICAD Polyclonal Antibody, Unconjugated(SL0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ICAD/DFF-45 Beta Polyclonal Antibody, Unconjugated(SL0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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