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Home > Product > Antibody > Rabbit Anti-IFN gamma antibody
IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_MOUSE; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon.
Cat:
SL096R
Species Reactivity：
Human,Mouse,Rat,
Immunogen：
KLH conjugated synthetic peptide derived from mouse IFN gamma:75-155/155
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Disease Lung (Mouse) Lysate at 30 ugDisease Spleen (Mouse) lysate at 30 ugPrimary: Anti- IFN gamma (SL096R) at 1/200 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/3000 dilutionPredicted band size: 15 kDObserved band size: 18 kDSample: Lane 1: Mouse Raw264.7-PMA Lysates Lane 2: Mouse Lymph Lysates Lane 3: Mouse Spleen Lysates Lane 4: Mouse Thymus Lysates Lane 5: Rat Lymph Lysates Lane 6: Rat Thymus Lysates Lane 7: Human Jurkat-TPA cell Lysates Lane 8: Human Jurkat cell Lysates Lane 9: Human HepG2 cell Lysates Lane 10: Recombinant human IFN gamma Protein (100ng) (SL0388P) Primary: Anti-IFN gamma (SL096R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 15kDaObserved band size: 25kDaTissue/cell: Mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated(SL096R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IFN-gamma Polyclonal Antibody, Unconjugated(SL096R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Mouse lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated(SL096R) 1:600, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei
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Product PDFs
Datasheet:


Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.

Function:
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Subunit:
Homodimer.

Subcellular Location:
Secreted.

Tissue Specificity:
Released primarily from activated T lymphocytes.

Post-translational modifications:
Proteolytic processing produces SLCterminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

DISEASE:
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

Similarity:
Belongs to the type II (or gamma) interferon family.

SWISS:
P0316

Gene ID:
15978

Database links:

Entrez Gene: 3458 Human

Entrez Gene: 15978 Mouse

Entrez Gene: 72697 Rat

Omim: 147570 Human

SwissProt: P01579 Human

SwissProt: P0316 Mouse

Unigene: 856 Human

Unigene: 48327 Mouse



Picture

Sample:
Disease Lung (Mouse) Lysate at 30 ug
Disease Spleen (Mouse) lysate at 30 ug
Primary: Anti- IFN gamma (SL096R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/3000 dilution
Predicted band size: 15 kD
Observed band size: 18 kD
Sample:
Lane 1: Mouse Raw264.7-PMA Lysates
Lane 2: Mouse Lymph Lysates
Lane 3: Mouse Spleen Lysates
Lane 4: Mouse Thymus Lysates
Lane 5: Rat Lymph Lysates
Lane 6: Rat Thymus Lysates
Lane 7: Human Jurkat-TPA cell Lysates
Lane 8: Human Jurkat cell Lysates
Lane 9: Human HepG2 cell Lysates
Lane 10: Recombinant human IFN gamma Protein (100ng) (SL0388P)
Primary: Anti-IFN gamma (SL096R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15kDa
Observed band size: 25kDa
Tissue/cell: Mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated(SL096R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IFN-gamma Polyclonal Antibody, Unconjugated(SL096R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Mouse lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated(SL096R) 1:600, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei
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