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Home > Product > Antibody > Rabbit Anti-IL-10 antibody
Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN.白介素10
Cat:
SL0698R
Species Reactivity：
Human,Mouse,(predicted: Rat,Chicken,Pig,Rabbit,Sheep,Guinea Pig,)
Immunogen：
KLH conjugated synthetic peptide derived from human IL-10:101-178/178
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Lane 1: THP-1 (Human) Cell Lysate at 30 ugLane 2: THP-1-LPS (Human) Cell Lysate at 30 ugPrimary: Anti-IL-10 (SL0698R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 18.6 kDObserved band size: 17 kDSample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-IL-10 (SL0698R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 18 kDObserved band size: 18 kDParaformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (SL0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB stainingTissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(SL0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(SL0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HISLV1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020]

Function:
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subunit:
Homodimer.

Subcellular Location:
Secreted.

Tissue Specificity:
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Similarity:
Belongs to the IL-10 family.

SWISS:
P22301

Gene ID:
3586

Database links:

Entrez Gene: 3586 Human

Entrez Gene: 16153 Mouse

Entrez Gene: 25325 Rat

Omim: 14892 Human

SwissProt: P22301 Human

SwissProt: P18893 Mouse

SwissProt: P29456 Rat

Unigene: 193717 Human

Unigene: 874 Mouse

Unigene: 9868 Rat



白细胞介素-10(IL-10)是主要由Th2细胞所产生的一种细胞因子，活化的B细胞、单核巨噬细胞和枯否氏细胞（Kupffer's cell）等也可产生IL-10。它是以抑制Th细胞克隆细胞因子合成为特点的多效免疫调节因子。
白介素-10是维护细胞因子网络平衡的重要负调节因子。其作用机制可能是降低抗原递呈细胞MHCⅡ类抗原表达，或诱导抗原递呈细胞产生另一种细胞因子，改变细胞内信号传递途径，从而选择性抑制某些细胞因子mRNA转录，并与Th2细胞产生的IL-4、IL-5有协同作用。 Picture

Sample:
Lane 1: THP-1 (Human) Cell Lysate at 30 ug
Lane 2: THP-1-LPS (Human) Cell Lysate at 30 ug
Primary: Anti-IL-10 (SL0698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18.6 kD
Observed band size: 17 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-IL-10 (SL0698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (SL0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(SL0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(SL0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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