Rabbit Anti-IL-1 Alpha Propeptide antibody

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interleukin-1 alpha; Hematopoietin 1; IL 1 alpha; IL1 alpha; IL 1; IL 1A; Il-1a; IL1; IL1A; IL1F1; ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; Preinterleukin 1 alpha; Pro interleukin 1 alpha; Prointerleukin 1 alpha; IL1A_

Cat:

SL0555R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse interleukin-1 alpha Propeptide:51-112/270

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Raw264.7(Mouse) Cell Lysate at 30 ugPrimary: Anti-IL-1 Alpha Propeptide (SL0555R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12/31 kDObserved band size: 31 kDSample: Raw264.7-LPS(Mouse) Cell Lysate at 10 ngRaw264.7-LPS(Mouse) Cell Lysate at 100 ngRaw264.7-LPS(Mouse) Cell Lysate at 1 ugPrimary: Anti-IL-1 Alpha Propeptide (SL0555R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12/31 kDObserved band size: 31 kDTissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-1 Alpha Polyclonal Antibody, Unconjugated(SL0555R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-1 Alpha Polyclonal Antibody, Unconjugated(SL0555R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Function:
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Subunit:
Monomer.

Subcellular Location:
Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Similarity:
Belongs to the IL-1 family.

SWISS:
P01582

Gene ID:
16175

Database links:

Entrez Gene: 3552 Human

Entrez Gene: 16175 Mouse

Entrez Gene: 24493 Rat

Omim: 147760 Human

SwissProt: P01583 Human

SwissProt: P01582 Mouse

SwissProt: P16598 Rat

Unigene: 1722 Human

Unigene: 15534 Mouse

Unigene: 12300 Rat

IL-1α主要由胶原细胞产生。 Picture

Sample:
Raw264.7-LPS(Mouse) Cell Lysate at 10 ng
Raw264.7-LPS(Mouse) Cell Lysate at 100 ng
Raw264.7-LPS(Mouse) Cell Lysate at 1 ug
Primary: Anti-IL-1 Alpha Propeptide (SL0555R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/31 kD
Observed band size: 31 kD

Tissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-1 Alpha Polyclonal Antibody, Unconjugated(SL0555R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-1 Alpha Polyclonal Antibody, Unconjugated(SL0555R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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