Rabbit Anti-ATPase Na+/ K+ beta 2 antibody

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adhesion molecule on glia; Na+K+ATPase; AMOG; AT1B2; AT1B2_HUMAN; ATP1B2; ATPase Na+/K+ transporting beta 2 polypeptide; ATPB2; ATPB2S; MGC93648; Na+/K+ -ATPase beta 2 subunit; Na, K ATPase beta 2 polypeptide; RATATPB2S; sodium potassium ATPase subunit be

Cat:

SL1152R

Species Reactivity：

Human,Mouse,(predicted: Rat,Pig,Cow,Horse,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human ATP1b2:201-290/290<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2ug/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Human K562 cell lysates Lane 3: Human SH-SY5Y cell lysates Primary: Anti-ATPase Na+/ K+ beta 2 (SL1152R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 33 kDaObserved band size: 60 kDaTissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated(SL1152R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated(SL1152R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $136.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. [provided by RefSeq, Jul 2008]

Function:
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.

Subunit:
Composed of three subunits: alpha (catalytic), beta and gamma.

Subcellular Location:
Membrane; Single-pass type II membrane protein.

Similarity:
Belongs to the X(+)/potassium ATPases subunit beta family.

SWISS:
P14415

Gene ID:
482

Database links:

Entrez Gene: 482 Human

Omim: 182331 Human

SwissProt: P14415 Human

Unigene: 643540 Human

通道蛋白（Channel Protein）
钠钾ATP酶是位于细胞膜上的一种糖蛋白,与ATP 的分解和细胞内外钠、钾离子的转运密切相关,哺乳动物各种组织细胞的钠钾ATP 酶的免疫学特性基本相同。 Picture

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated(SL1152R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated(SL1152R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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