Rabbit Anti-MyD88 antibody | Sunlong Medical

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myeloid differential protein-88; Myeloid differentiation primary response gene; MYD 88; MYD88D; Myeloid differentiation marker 88; Myeloid differentiation primary response gene 88; Myeloid differentiation primary response gene; Myeloid differentiation pri

Cat:

SL1047R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from mouse MyD88:201-296/296

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Molt-4 Cell (Human) Lysate at 40 ugPrimary: Anti-MyD88 (SL1047R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDObserved band size: 36 kDTissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MyD88 Polyclonal Antibody, Unconjugated(SL1047R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingParaformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MyD88) Polyclonal Antibody, Unconjugated (SL1047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MyD88) Polyclonal Antibody, Unconjugated (SL1047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HO-1) Polyclonal Antibody, Unconjugated (SL0827R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-MyD88 antibody (SL1047R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a SLCterminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010].

Function:
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.

Subunit:
Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1 (By similarity). Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. May interact with PIK3AP1 (By similarity). Upon IL1B treatment, forms a complex with PELI1, IRAK1, IRAK4 and TRAF6; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitous.

DISEASE:
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.

Similarity:
Contains 1 death domain.
Contains 1 TIR domain.

SWISS:
P22366

Gene ID:
17874

Database links:

Entrez Gene: 4615 Human

Entrez Gene: 17874 Mouse

Entrez Gene: 301059 Rat

Omim: 602170 Human

SwissProt: Q99836 Human

SwissProt: P22366 Mouse

SwissProt: Q6Y1S1 Rat

Unigene: 82116 Human

Unigene: 213003 Mouse

Unigene: 37341 Rat

MyD88是天然免疫中的调控分子,可能在感染、炎症、免疫等病理生理过程中具有更广泛的生物学功能,　MyD88蛋白是由Toll样受体介导的先天免疫应答反应中重要的胞浆接头蛋白，由它参与构成的信号级联最终引起NF-kB依赖性信号通路的活化。 Picture

Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MyD88 Polyclonal Antibody, Unconjugated(SL1047R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MyD88) Polyclonal Antibody, Unconjugated (SL1047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MyD88) Polyclonal Antibody, Unconjugated (SL1047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HO-1) Polyclonal Antibody, Unconjugated (SL0827R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-MyD88 antibody (SL1047R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (30) | SL1047R has been referenced in 30 publications.

[IF=3.69] WB ; rat.

PubMed:32810620

[IF=2.952] Yongfeng Zhang. et al. Protection against ulcerative colitis and colorectal cancer by evodiamine via anti‑inflammatory effects. Mol Med Rep. 2022 May;25(5):1-14 WB ; Mouse.

PubMed:35362542

[IF=2.563] Xu D et al. Saturated Fatty Acids Activate the Inflammatory Signalling Pathway in Schwann Cells: Implication in Sciatic Nerve Injury. Scand J Immunol. 2020 Jun 17;e12896. WB ELISA IHSLCP Flow-Cyt ; Rabbit,Rat.

PubMed:32557749

[IF=4.35] Yun-Jiao Shi. et al. Impact of the Plastein Reaction of Casein Hydrolysates in the Presence of Exogenous Amino Acids on Their Anti-Inflammatory Effect in the Lipopolysaccharide-Stimulated Macrophages. Foods. 2022 Jan;11(2):196 WB ; Mouse.

PubMed:35053927

[IF=3.524] Rossetto Isabela Maria Urra. et al. Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum. Toxicol Res-Uk. 2021 Aug;10(4):835-849 WB ; Rat.

PubMed:34484675

[IF=3.69] Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-κB and TGF-β/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275 WB ; Rat.

PubMed:32810620

[IF=1.813] Li Li. et al. Acupuncture at Back-Shu and Front-Mu Acupoints Prevents Gastric Ulcer by Regulating the TLR4/MyD88/NF-κB Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8214052 WB ; Rat.

PubMed:33628315

[IF=2.717] Xu D et al. Saturated fatty acids activate the inflammatory signalling pathway in Schwann cells: Implication in sciatic nerve injury. Scand J Immunol . 2020 Aug;92(2):e12896. WB ; Rat.

PubMed:32557749

[IF=3.414] Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361. WB ; Human.

PubMed:31683033

[IF=4.868] Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324. WB ; Mouse.

PubMed:31182999

[IF=2.553] Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63. IHC ; Mouse.

PubMed:31073286

[IF=1.774] Wang X et al. Pretreatment with Salvia miltiorrhiza Polysaccharides Protects from Lipopolysaccharides/d-Galactosamine-Induced Liver Injury in Mice through Inhibiting TLR4/MyD88 Signaling Pathway. J Interferon Cytokine Res. 2019 May 10. WB ; Mouse.

PubMed:31074668

[IF=3.82] Fu, Juanli, et al. "The acute exposure of tetrachloro-p-benzoquinone (aka chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning." Toxicology (2017). WB ; Rat.

PubMed:28238930

[IF=1.92] Wang, Dunjing, et al. "Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-κB Activation in Microglial Cells." Inflammation: 1-8. WB ; Mouse.

PubMed:26002587

[IF=5.875] Tengteng Yuan. et al. PF-PLC micelles ameliorate cholestatic liver injury via regulating TLR4/MyD88/NF-κB and PXR/CAR/UGT1A1 signaling pathways in EE-induced rats. Int J Pharmaceut. 2022 Mar;615:12296 WB ; Rat.

PubMed:35041917

[IF=5.31] Xueqing Hu. et al. Tacrolimus alleviates LPS-induced AKI by inhibiting TLR4/MyD88/NF-κB signalling in mice. 2021 Dec 09 WB ; Mouse.

PubMed:34889045

[IF=2.967] Lili Li. et al. Protein overexpression of toll‑like receptor 4 and myeloid differentiation factor 88 in oral squamous cell carcinoma and clinical significance. Oncol Lett. 2021 Nov;22(5):1-11 IHC ; human.

PubMed:34594427

[IF=4.162] Jiali Yang. et al. Baicalin Rescues Cognitive Dysfunction, Mitigates Neurodegeneration, and Exerts Anti-Epileptic Effects Through Activating TLR4/MYD88/Caspase-3 Pathway in Rats. Drug Des Dev Ther. 2021 Jul;15:3163-336 IHC ; Rat.

PubMed:34321866

[IF=2.1] Xuemei Zhang. et al. Resveratrol affects the expression of uric acid transporter by improving inflammation. Mol Med Rep. 2021 Aug;24(2):1-9 WB ; Mouse.

PubMed:34109437

[IF=9.038] Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:14850 WB ; Human.

PubMed:33053467

[IF=5.085] Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441

PubMed:33552047

[IF=4.486] Shun Huang. et al. Design, Synthesis, and Activity Study of Cinnamic Acid Derivatives as Potent Antineuroinflammatory Agents. Acs Chem Neurosci. 2021;12(3):419–429 WB ; Mouse.

PubMed:33439002

[IF=5.162] Liu S et al. Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells. Int J Biol Macromol . 2020 Oct 15;161:797-809. WB ; Mouse.

PubMed:32553971

[IF=3] Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923. WB ; Chicken.

PubMed:doi:10.2147/IDR.S231908

[IF=0.417] Han C et al. Modulation of TLR2 and TLR4 in macrophages following Trichinella spiralis infection.Helminthologia, 55(3), 195–203. WB ; Mouse.

PubMed:10.2478/helm-2018-0015

[IF=1.58] Song, Fang, et al. "Correlation of TLR4/MyD88 signaling with early miscarriage." International Journal of Clinical and Experimental Pathology 10.3 (2017): 721-728. IHSLCP ; Human.

PubMed:ISSN:1936-2625/IJCEP0042168

[IF=4.26] Li, Chengcheng, et al. "Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway." Scientific Reports 7 (2017). WB ; Mouse.

PubMed:28725012

[IF=3.26] Chen, Gangling, et al. "Limb Remote Ischemic Postconditioning Reduces Ischemia-Reperfusion Injury by Inhibiting NADPH Oxidase Activation and MyD88-TRAF6-P38MAP-Kinase Pathway of Neutrophils." International Journal of Molecular Sciences 17.12 (2016): 1971. WB ; Rat.

PubMed:27819607

[IF=3.88] Fu, Juanli, et al. "Tetrachlorobenzoquinone exerts neurological pro-inflammatory activity by promoting HMGB1 release, which induces TLR4 clustering within the lipid raft." Toxicological Sciences (2016): kfw124. WB, IP ; Rat.

PubMed:27413111

[IF=1.72] Lu, Na-na, et al. "Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1." Evidence-Based Complementary and Alternative Medicine 2014 (2014). WB ; Mouse.

PubMed:24527057