Rabbit Anti-Nociceptin receptor antibody

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H.sapiens mRNA for ORL1 receptor; Kappa type 3 opioid receptor; Kappa-type 3 opioid receptor; KOR 3; KOR-3; KOR3; LC132 receptor like; MGC34578; Nociceptin receptor; NOCIR; OOR; Opiate receptor like 1; Opiod receptor like 1; Oprl1; OPRX_HUMAN; ORL1; Orpha

Cat:

SL0181R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human Nociceptin receptor:1-47/370<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart(Rat) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-Nociceptin receptor (SL0181R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kDObserved band size : 40kDSample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- Nociceptin receptor (SL0181R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 52 kD免疫组化（IHC）ImmunohistochemistryTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(SL0181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining免疫荧光Immunofluorescence组织切片：Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(SL0181R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Nociceptin receptor Receptor for the neuropeptide nocipeptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase[subcellular location] integral membrane protein. Belongs to the G-protein coupled receptor 1 family.

Function:
Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
In the brain, isoform KOR3 and isoform KOR3C are most abundant in hypothalamus and periaqueductal gray. Isoform KOR3A is highly expressed in cortex, striatum and brainstem. Isoform KOR3D is highly expressed in cerebellum, hypothalamus and brainstem.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P35377

Gene ID:
4987

Database links:

Entrez Gene: 4987 Human

Entrez Gene: 18389 Mouse

Entrez Gene: 29256 Rat

Omim: 602548 Human

SwissProt: P41146 Human

SwissProt: P35377 Mouse

SwissProt: P35370 Rat

Unigene: 2859 Human

Unigene: 285075 Mouse

Unigene: 9762 Rat

Picture

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Nociceptin receptor (SL0181R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 52 kD

免疫组化（IHC）Immunohistochemistry Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(SL0181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

免疫荧光Immunofluorescence 组织切片： Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(SL0181R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

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